Anti-ROCK1 Rabbit Monoclonal Antibody

Product Name

Anti-ROCK1 Rabbit Monoclonal Antibody

View all ROCK1 Antibodies

SKU/Catalog Number

M00722

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ROCK1 Rabbit Monoclonal Antibody catalog # M00722. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ROCK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00722)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CDE-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ROCK1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00722 is reactive to ROCK1 in Human, Mouse, Rat

Applications

M00722 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

170 kDa

Calculated molecular weight

158.175kDa

Background of ROCK1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FROCK1 using anti-ROCK1 antibody (M00722).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified monoclonal antibody (Catalog # M00722) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 170 kDa. The expected band size for ROCK1 is at 158 kDa.

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Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody(M00722)
ROCK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROCK1 Antibody (M00722)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:150 dilution.

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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:150 dilution.

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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:150 dilution.

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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:150 dilution.

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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:150 dilution.

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Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For ROCK1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.1; MGC131603; MGC43611; p160 ROCK-1; p160ROCK; p160-ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; ROCK1; ROK beta ROCK1 P160ROCK, ROCK-I Rho associated coiled-coil containing protein kinase 1 rho-associated protein kinase 1|p160 ROCK-1|renal carcinoma antigen NY-REN-35

For more info on ROCK1, check out the ROCK1 Infographic

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In this infographic, you will see the following information for ROCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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