Anti-RRM2 Monoclonal Antibody

Product Name

Anti-RRM2 Monoclonal Antibody

View all RRM2 Antibodies

SKU/Catalog Number

M01978

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-RRM2 Monoclonal Antibody catalog # M01978. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RRM2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01978)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAE-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human RRM2 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01978 is reactive to RRM2 in Human

Applications

M01978 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

44.878kDa

Background of RRM2

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RRM2 using anti-RRM2 antibody (M01978).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified monoclonal antibody (Catalog # M01978) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 45 kDa. The expected band size for RRM2 is at 45 kDa.

Gene/Protein Information For RRM2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.17.4.1; R2; ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase M2 polypeptide; ribonucleotide reductase M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RR2; RR2M RRM2 C2orf48, R2, RR2, RR2M ribonucleotide reductase regulatory subunit M2 ribonucleoside-diphosphate reductase subunit M2|ribonucleotide reductase M2 polypeptide|ribonucleotide reductase small chain|ribonucleotide reductase small subunit|uncharacterized protein C2orf48

For more info on RRM2, check out the RRM2 Infographic

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In this infographic, you will see the following information for RRM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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