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Product Info Summary
| SKU: | M04507 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-SCAP Antibody (N-term)
SKU/Catalog Number
M04507
Size
50 µl
Description
Boster Bio Anti-SCAP Antibody (N-term) (Catalog # M04507). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SCAP Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04507)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1992C245.37.16
Isotype
IgG1,κ
Immunogen
This SCAP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the N-terminal region of human SCAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04507 is reactive to SCAP in Human
Applications
M04507 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
139.729kDa
Background of SCAP
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-SCAP Antibody (N-term) at 1:4000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: LNCaP whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 140 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For SCAP (Source: Uniprot.org, NCBI)
Gene Name
SCAP
Full Name
Sterol regulatory element-binding protein cleavage-activating protein
Weight
139.729kDa
Superfamily
WD repeat SCAP family
Alternative Names
KIAA0199; PSEC0227; SREBF chaperone; SREBP cleavage-activating protein; sterol regulatory element binding protein cleavage-activating protein SCAP SREBF chaperone sterol regulatory element-binding protein cleavage-activating protein|SREBP cleavage-activating protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCAP, check out the SCAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCAP Antibody (N-term) (M04507)
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No publications found for M04507
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Customer Reviews
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