Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody

Product Name

Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody

View all Sephs2 Antibodies

SKU/Catalog Number

P11872

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody (Catalog # P11872). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Selenophosphate Synthetase 2 SEPHS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P11872)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P11872 is reactive to Sephs2 in Mouse

Applications

P11872 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

47.834kDa

Background of Sephs2

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase (SelD) catalyzes the conversion of selenium to selenophosphate which is required by a number of bacterial, archaeal and eukaryotic organisms for synthesis of selenocysteine-tRNA, the precursor of selenocysteine in selenoenzymes. A second selenophosphate synthetase (SPS2) was identified in mammals. SPS2 is itself a selenoprotein in mammals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot using Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

Gene/Protein Information For Sephs2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.9.3; Selenophosphate synthase 2; selenophosphate synthetase 2; SPS2water dikinase 2 Sephs2|S, Sps2, Ysg, Ysg3|selenophosphate synthetase 2|selenide, water dikinase 2|selenium donor protein 2|selenophosphate synthase 2|the SelD gene product

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