Anti-SERCA2 ATPase/ATP2A2 Antibody
Rabbit IgG polyclonal antibody for Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(ATP2A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s). Independently reviewed in 1 review(s).
Anti-SERCA2 ATPase/ATP2A2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
FCGR3B, also known as CD16b belongs to the Ig super family. FCGR3B encodes a glycosylphosphatidylinositol (GPI)-anchored protein which is expressed constitutively by neutrophils and after gamma-interferon stimulation by eosinophils. FCGR3B binds to complex or aggregated IgG and monomeric IgG. Different from III-A, FCGR3B cannot arbitrate antibody-dependent cytotoxicity and phagocytosis. FCGR3B operate to capture immune complexes in peripheral blood circulation that does not activate neutrophils.
|Product Name||Anti-SERCA2 ATPase/ATP2A2 Antibody
See all SERCA2 ATPase primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for 1258 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. 1258 information: Molecular Weight: 114757 MW; Subcellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein; Tissue Specificity: Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.|
|Cite This Product||Anti-SERCA2 ATPase/ATP2A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1720)|
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|Anti-SERCA2 ATPase/ATP2A2 Antibody may replace the following items: sc 271669|sc 30110|sc 376235|sc 53010|sc 73022|sc 8094|sc 8095.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase(665-679aa QRDACLNARCFARVE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ATP2A2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||488|
|Species Of This Entry||Human|
|Protein Name||Sarcoplasmic/endoplasmic reticulum calcium ATPase 2|
|Superfamily||cation transport ATPase (P-type) (TC 3.A.3) family|
|Alternative Names||SERCA2 ATPase|ATP2BFLJ20293; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform; cardiac Ca2+ ATPase; DAR; DD; EC 3.6.3; EC 22.214.171.124; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; FLJ38063; MGC45367; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2DKFZp686P0211; SR Ca(2+)-ATPase 2|
|Post Tranlational Modifications||Phosphorylation; Oxidation; Methylation; Dephosphorylation; Nitration; Reduction; Cleavage; Sumoylation; Demethylation; Nitrosylation; Hydroxylation; Acetylation|
|Subcellular Localization||Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.|
|Gene Location||12q24.11, on Chromasome 12, gene sequence: NC_000012.12 (110281247..110351093)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ATP2A2 (Source: Uniprot.org, NCBI)
|Protein Function||This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11- induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (By similarity).|
|Tissue Specificity||Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.|
|Interacting Proteins||CFTR; F2RL1; OPRD1|
|Related Diseases||Keratosis Follicularis; Heart Failure; Endometriosis, Site Unspecified; Hypertrophy; Cardiomyopathies; Dermatologic Disorders; Cardiac Hypertrophy; Diabetes Mellitus; Myocardial Infarction; Infarction; Acantholysis; Tissue Adhesions; Fibrosis; Skin Diseases, Genetic; Ventricular Dysfunction, Left; Malignant Neoplasms; Hypertensive Disease; Left Ventricular Hypertrophy; Cardiomyopathy, Dilated|
|Related Pathways||Transport; Keratinization; Localization; Pathogenesis; Secretory Pathway; Aging; Cell Death; Secretion; Glycolysis; Keratinocyte Differentiation; Methylation; Cell Growth; Exocytosis; Gene Silencing; Sensitization; Translation; Cell Adhesion; Swimming; Cell Proliferation; Dna Methylation|
|Background||SERCA2(SARCOPLASMIC RETICULUM Ca(2+)-ATPase 2), also called ATP2A2, ATP2B, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca(2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped on 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression.SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells. Perilesional Darier disease skin was comparable to normal skin.|
|Scientific References||PMID: 2844796 by Lytton J., et al. Molecular cloning of cDNAs from human kidney coding for two alternatively spliced products of the cardiac Ca2+-ATPase gene.|
PMID: 12659872 by Gelebart P., et al. Identification of a new SERCA2 splice variant regulated during monocytic differentiation.
Our Boster Quality Guarantee for Anti-SERCA2 ATPase/ATP2A2 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Human Mammary Cancer Tissue
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
|Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...||PubMed ID 24324360|
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