Anti-SERCA2 ATPase/ATP2A2 Antibody

Product Name

Anti-SERCA2 ATPase/ATP2A2 Antibody

View all SERCA2 ATPase Antibodies

SKU/Catalog Number

PA1720

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SERCA2 ATPase/ATP2A2 Antibody catalog # PA1720. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SERCA2 ATPase/ATP2A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1720)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1720 is reactive to ATP2A2 in Human, Mouse, Rat

Applications

PA1720 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

114.757kDa

Background of SERCA2 ATPase

SERCA2 (SARCOPLASMIC RETICULUM Ca (2+)-ATPase 2), also called ATP2A2, ATP2B, encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca (2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped on 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression.SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells. Perilesional Darier disease skin was comparable to normal skin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-SERCA2 ATPase antibody, PA1720, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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Anti-SERCA2 ATPase antibody, PA1720, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate

Gene/Protein Information For ATP2A2 (Source: Uniprot.org, NCBI)

Alternative Names

ATP2BFLJ20293; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform; cardiac Ca2+ ATPase; DAR; DD; EC 3.6.3; EC 3.6.3.8; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; FLJ38063; MGC45367; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2DKFZp686P0211; SR Ca(2+)-ATPase 2 ATP2A2 ATP2B, DAR, DD, SERCA2 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 sarcoplasmic/endoplasmic reticulum calcium ATPase 2|ATPase Ca++ transporting cardiac muscle slow twitch 2|ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2|SR Ca(2+)-ATPase 2|calcium pump 2|cardiac Ca2+ ATPase|endoplasmic reticulum class 1/2 Ca(2+) ATPase

For more info on ATP2A2, check out the ATP2A2 Infographic

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In this infographic, you will see the following information for ATP2A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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