Anti-Setdb2 Antibody

Product Name

Anti-Setdb2 Antibody

View all SETDB2 Antibodies

SKU/Catalog Number

A08290

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Setdb2 Antibody (Catalog # A08290). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Setdb2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08290)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08290 is reactive to SETDB2 in Human, Mouse

Applications

A08290 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

81.894kDa

Background of SETDB2

Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
WB: 1:10,000 - 1:100,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Setdb2 using anti-Setdb2 antibody (A08290).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Setdb2 antigen affinity purified polyclonal antibody (Catalog # A08290) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Setdb2.

Gene/Protein Information For SETDB2 (Source: Uniprot.org, NCBI)

Alternative Names

C13orf4; chromosome 13 open reading frame 4; chronic lymphocytic leukemia deletion region 8; Chronic lymphocytic leukemia deletion region gene 8 protein; CLLD8DKFZp761J1217; DKFZp586I0123; EC 2.1.1.43; histone-lysine N-methyltransferase SETDB2; KMT1FCLLL8; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SET domain, bifurcated 2 SETDB2 C13orf4, CLLD8, CLLL8, KMT1F SET domain bifurcated histone lysine methyltransferase 2 histone-lysine N-methyltransferase SETDB2|SET domain bifurcated 2|chronic lymphocytic leukemia deletion region gene 8 protein|lysine N-methyltransferase 1F

For more info on SETDB2, check out the SETDB2 Infographic

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In this infographic, you will see the following information for SETDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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