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Product Info Summary
| SKU: | A10841-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SHCD/SHC4 Antibody Picoband™
SKU/Catalog Number
A10841-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHCD/SHC4 Antibody Picoband™ catalog # A10841-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SHCD/SHC4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10841-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SHCD/SHC4 recombinant protein (Position: Q57-Q519).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10841-1 is reactive to Shc4 in Human, Mouse, Rat
Applications
A10841-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.519kDa
Background of Shc4
Predicted to enable receptor tyrosine kinase binding activity. Predicted to be involved in transmembrane receptor protein tyrosine kinase signaling pathway. Predicted to act upstream of or within several processes, including apoptotic process; positive regulation of cell population proliferation; and stem cell differentiation. Predicted to be located in postsynaptic membrane. Predicted to be active in plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SHCD/SHC4 using anti-SHCD/SHC4 antibody (A10841-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCD/SHC4 antigen affinity purified polyclonal antibody (Catalog # A10841-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCD/SHC4 at approximately 69 kDa. The expected band size for SHCD/SHC4 is at 69 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Shc4 (Source: Uniprot.org, NCBI)
Gene Name
Shc4
Full Name
SHC-transforming protein 4
Weight
68.519kDa
Alternative Names
hShcD; MGC34023; Rai-like protein; RaLPSH2 domain protein C4; SHC (Src homology 2 domain containing) family, member 4; SHCD; SHC-transforming protein 4; SHC-transforming protein D; Src homology 2 domain-containing-transforming protein C4 Shc4|6230417E10Rik, 9930029B02Rik, Gm685, RaLP|SHC (Src homology 2 domain containing) family, member 4|SHC-transforming protein 4|SH2 domain protein C4|SHC-transforming protein D|mShcD|novel phosphotyrosine interaction domain (PTB/PID) and SH2 domains containing protein|rai-like protein RaLP|src homology 2 domain-containing-transforming protein C4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Shc4, check out the Shc4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Shc4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHCD/SHC4 Antibody Picoband™ (A10841-1)
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No publications found for A10841-1
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