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Product Info Summary
| SKU: | A07561 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SKA3 Antibody
SKU/Catalog Number
A07561
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SKA3 Antibody (Catalog # A07561). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SKA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SKA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07561)
Host
Rabbit
Contents
SKA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Reactive Species
A07561 is reactive to Ska3 in Human, Mouse, Rat
Applications
A07561 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
45.37kDa
Background of Ska3
Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Ska3 (Source: Uniprot.org, NCBI)
Gene Name
Ska3
Full Name
Spindle and kinetochore-associated protein 3
Weight
45.37kDa
Superfamily
SKA3 family
Alternative Names
spindle and kinetochore associated complex subunit 3; spindle and kinetochore-associated protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ska3, check out the Ska3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ska3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKA3 Antibody (A07561)
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No publications found for A07561
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Customer Reviews
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