Anti-SLC2A8 Antibody (Center)

Product Name

Anti-SLC2A8 Antibody (Center)

View all Glucose Transporter GLUT8 Antibodies

SKU/Catalog Number

M06641

Size

50 µl

Description

Boster Bio Anti-SLC2A8 Antibody (Center) (Catalog # M06641). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SLC2A8 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06641)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SLC2A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-292 amino acids from the Central region of human SLC2A8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06641 is reactive to SLC2A8 in Human, Mouse, Rat

Applications

M06641 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.819kDa

Background of Glucose Transporter GLUT8

Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:25
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-SLC2A8 Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: human testis lysate
Lane 4: Caco2 whole cell lysate
Lane 5: mouse testis lysate
Lane 6: rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M06641 staining SLC2A8 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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Overlay histogram showing U-2 OS cells stained with M06641 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M06641, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For SLC2A8 (Source: Uniprot.org, NCBI)

Alternative Names

GLUT-8; GLUT8solute carrier family 2 (facilitated glucose transporter) member 8; GLUTX1facilitated glucose transporter member 8; solute carrier family 2 (facilitated glucose transporter), member 8 SLC2A8 GLUT8, GLUTX1 solute carrier family 2 member 8 solute carrier family 2, facilitated glucose transporter member 8|glucose transporter type 8|glucose transporter type X1|solute carrier family 2 (facilitated glucose transporter), member 8

For more info on SLC2A8, check out the SLC2A8 Infographic

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In this infographic, you will see the following information for SLC2A8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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