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Product Info Summary
| SKU: | M01625 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-SNAP25 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01625
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SNAP25 Rabbit Monoclonal Antibody catalog # M01625. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAP25 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01625)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABHE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SNAP25
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01625 is reactive to SNAP25 in Human, Mouse, Rat
Applications
M01625 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
23.315kDa
Background of SNAP25
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of SNAP25 expression in SH-SY5Y cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SNAP25 (Source: Uniprot.org, NCBI)
Gene Name
SNAP25
Full Name
Synaptosomal-associated protein 25
Weight
23.315kDa
Superfamily
SNAP-25 family
Alternative Names
bA416N4.2; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP25; SNAP-25SNAP; SUP; Super protein; Synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa SNAP25 CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2 synaptosome associated protein 25 synaptosomal-associated protein 25|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein, 25kDa|synaptosome associated protein 25kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNAP25, check out the SNAP25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNAP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNAP25 Rabbit Monoclonal Antibody (M01625)
Hello CJ!
M01625 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
M01625 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:250
Zhong ZQ, Xiang Y, Hu X, Wang YC, Zeng X, Wang XM, Xia QJ, Wang TH, Zhang X. Neural Regen Res. 2017 Jun;12(6):969-976. doi: 10.4103/1673-5374.208592. Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomo...
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-SNAP25 Rabbit Monoclonal Antibody
Question
Is this M01625 anti-SNAP25 Rabbit Monoclonal antibody reactive to the isotypes of SNAP25?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of M01625 anti-SNAP25 Rabbit Monoclonal antibody is A synthesized peptide derived from human SNAP25. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
I have attached the WB image, lot number and protocol we used for fetal brain temporal cortex using anti-SNAP25 Rabbit Monoclonal antibody M01625. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
We ordered your anti-SNAP25 Rabbit Monoclonal antibody for IP on brain in the past. I am using mouse, and I plan to use the antibody for WB next. My question regards examining brain as well as fetal brain temporal cortex in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
I took a look at the website and datasheets of our anti-SNAP25 Rabbit Monoclonal antibody and it seems that M01625 has been validated on mouse in both IP and WB. Thus M01625 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-08
Question
Is there a BSA free version of anti-SNAP25 Rabbit Monoclonal antibody M01625 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 Rabbit Monoclonal antibody M01625 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-SNAP25 Rabbit Monoclonal antibody for WB for rat fetal brain temporal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain temporal cortex identification?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
As indicated on the product datasheet, M01625 anti-SNAP25 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain temporal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
We have been able to see staining in human skeletal muscle. Are there any suggestions? Is anti-SNAP25 Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
From literature skeletal muscle does express SNAP25. From Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2019-04-24
Question
I see that the anti-SNAP25 Rabbit Monoclonal antibody M01625 works with WB, what is the protocol used to produce the result images on the product page?
D. Brown
Verified customer
Asked: 2019-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain temporal cortex using anti-SNAP25 Rabbit Monoclonal antibody M01625. Let me know if you need anything else.
E. Kulkarni
Verified customer
Asked: 2019-03-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-04
Question
We are currently using anti-SNAP25 Rabbit Monoclonal antibody M01625 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The anti-SNAP25 Rabbit Monoclonal antibody (M01625) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-04
Question
I have a question about product M01625, anti-SNAP25 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Krishna
Verified customer
Asked: 2018-07-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01625 anti-SNAP25 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-04
Question
Does M01625 anti-SNAP25 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
You can see on the product datasheet, M01625 anti-SNAP25 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
I am interested in to test anti-SNAP25 Rabbit Monoclonal antibody M01625 on rat fetal brain temporal cortex for research purposes, then I may be interested in using anti-SNAP25 Rabbit Monoclonal antibody M01625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
The products we sell, including anti-SNAP25 Rabbit Monoclonal antibody M01625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-19
Question
We were well pleased with the WB result of your anti-SNAP25 Rabbit Monoclonal antibody. However we have observed positive staining in eye perinuclear region using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?
V. Roberts
Verified customer
Asked: 2015-09-09
Answer
According to literature, eye does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2015-09-09
Question
Does anti-SNAP25 Rabbit Monoclonal antibody M01625 work for WB with fetal brain temporal cortex?
Z. Walker
Verified customer
Asked: 2015-06-11
Answer
According to the expression profile of fetal brain temporal cortex, SNAP25 is highly expressed in fetal brain temporal cortex. So, it is likely that anti-SNAP25 Rabbit Monoclonal antibody M01625 will work for WB with fetal brain temporal cortex.
Boster Scientific Support
Answered: 2015-06-11
Question
Is a blocking peptide available for product anti-SNAP25 Rabbit Monoclonal antibody (M01625)?
T. Li
Verified customer
Asked: 2014-02-07
Answer
We do provide the blocking peptide for product anti-SNAP25 Rabbit Monoclonal antibody (M01625). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-02-07
