Anti-Sts-1 UBASH3B Antibody

Product Name

Anti-Sts-1 UBASH3B Antibody

View all UBASH3B/STS1/Tula-2 Antibodies

SKU/Catalog Number

A09570

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Sts-1 UBASH3B Antibody (Catalog # A09570). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Sts-1 UBASH3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09570)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of mouse Sts-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09570 is reactive to Ubash3b in Human, Mouse

Applications

A09570 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

71.443kDa

Background of UBASH3B/STS1/Tula-2

Sts-1 is a protein that inhibits endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. Sts-1 and Sts-2 (formerly p70 and Clip4, respectively) have been found to interact with Cbl, an ubiquitin ligase that plays a critical role in attenuation of receptor tyrosine kinase signaling by inducing ubiquitination and promoting their sorting for endosomal degradation. Sts-1 and Sts-2 contain SH3 domains that interact with Cbl, Ub-associated domains, which bind directly to mono-Ub or to the EGFR/Ub chimera, as well as phosphoglycerate mutase domains that mediate oligomerization of Sts-1/2. Sts-1 and Sts-2 also have been found to negatively regulate signaling pathways that control T cell receptors, which in turn affect the extent and duration of the T cell response to foreign pathogens.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: 4µg/ml
IF Microscopy: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Sts-1 was detected in paraffin-embedded sections of histiocytic elements in cytoplasm of human tonsil at 20x and 40x (B & C) using rabbit anti-Sts-1 Antigen Affinity purified polyclonal antibody (Catalog # A09570) at 4 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Western blot analysis of Sts-1 expression in recombinant (truncated) Sts-1-GST (lane 1). Sts-1 at 70KD was detected using rabbit anti-Sts-1 Antigen Affinity purified polyclonal antibody (Catalog # A09570) at 0.3 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For Ubash3b (Source: Uniprot.org, NCBI)

Alternative Names

Cbl-interacting Protein p70; Cbl-interacting protein Sts-1; KIAA1959SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling1, nm23-phosphorylated unknown substrate; MGC15437; nm23-phosphorylated unknown substrate; p70; STS1; STS1EC 3.1.3.48; STS-1ubiquitin-associated and SH3 domain-containing protein B; Suppressor of T-cell receptor signaling 1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2; UBASH3B; ubiquitin associated and SH3 domain containing B Ubash3b|2810457I06Rik, BB125008, STS-1, Sts, TULA, TULA-2, p70|ubiquitin associated and SH3 domain containing, B|ubiquitin-associated and SH3 domain-containing protein B|T-cell ubiquitin ligand 2|cbl-interacting protein p70|suppressor of T-cell receptor signaling 1|tyrosine-protein phosphatase STS1/TULA2

For more info on Ubash3b, check out the Ubash3b Infographic

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In this infographic, you will see the following information for Ubash3b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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