Anti-SUMO3 Antibody

Product Name

Anti-SUMO3 Antibody

View all SUMO3 Antibodies

SKU/Catalog Number

A05193

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SUMO3 Antibody (Catalog # A05193). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SUMO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SUMO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05193)

Host

Rabbit

Contents

SUMO3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SUMO3 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human SUMO3. The immunogen is located within the last 50 amino acids of SUMO3.

Reactive Species

A05193 is reactive to SUMO3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

11.6 kDa

Background of SUMO3

Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05193 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SUMO3 antibody can be used for detection of SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SUMO3

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