Anti-Antigen peptide transporter 1 TAP1 Antibody

Product Name

Anti-Antigen peptide transporter 1 TAP1 Antibody

View all TAP1 Antibodies

SKU/Catalog Number

A01123

Size

100µl

Form

Liquid. Whole rabbit serum.

Description

Boster Bio Anti-Antigen peptide transporter 1 TAP1 Antibody catalog # A01123. Tested in IP, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Antigen peptide transporter 1 TAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01123)

Host

Rabbit

Contents

Liquid. Whole rabbit serum.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to a portion of human TAP1 .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01123 is reactive to TAP1 in Human, Rat

Applications

A01123 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

87.218kDa

Background of TAP1

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Scanlan M.J., Cancer Immun. 1:4-4(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Baas A.F., EMBO J. 22:3062-3072(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunoprecipitation (1:100)
Western Blot (ECL, 1:1,000-1:4000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 2. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 3. IHC analysis of TAP1 using anti-TAP1 antibody (A01123).
TAP1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAP1 Antibody (A01123) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. Western blot analysis of TAP1 using anti-TAP1 antibody (A01123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP1 antigen affinity purified polyclonal antibody (Catalog # A01123) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TAP1.

Gene/Protein Information For TAP1 (Source: Uniprot.org, NCBI)

Alternative Names

ABC Transporter, MHC 1; ABC17; ABCB2; ABCB2FLJ41500; antigen peptide transporter 1; APT1; ATP-binding cassette sub-family B member 2; ATP-binding cassette, sub-family B (MDR/TAP), member 2; D6S114E; Ham1; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF1; Peptide Transporter TAP1; PSF1; PSF-1; PSF1ABC17; Really interesting new gene 4 protein; RING4; RING4FLJ26666; TAP1; TAP1*0102N; TAP1N; transporter 1, ATP-binding cassette, sub-family B (MDR/TAP); transporter associated with antigen processing; transporter, ATP-binding cassette, major hist TAP1 ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4*0102N, TAP1N, TAP1 transporter 1, ATP binding cassette subfamily B member antigen peptide transporter 1|ABC transporter, MHC 1|ATP-binding cassette sub-family B member 2|ATP-binding cassette, sub-family B (MDR/TAP), member 2|peptide supply factor 1|peptide transporter PSF1|peptide transporter TAP1|peptide transporter involved in antigen processing 1|really interesting new gene 4 protein|transporter 1 ATP-binding cassette sub-family B|transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|transporter associated with antigen processing|transporter, ATP-binding cassette, major histocompatibility complex, 1

For more info on TAP1, check out the TAP1 Infographic

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In this infographic, you will see the following information for TAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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