Anti-TAZ Antibody

Product Name

Anti-TAZ Antibody

View all TAZ/WWTR1 Antibodies

SKU/Catalog Number

A02672

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TAZ Antibody (Catalog # A02672). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TAZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02672)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

5H2

Isotype

IgG

Immunogen

Peptide sequence around aa.386~390 (V-E-S-A-L) derived from Human TAZ.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02672 is reactive to WWTR1 in Human, Mouse, Rat

Applications

A02672 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

44.101kDa

Background of TAZ/WWTR1

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Kanai F., Marignani P.A., Sarbassova D., Yagi R. EMBO J. 19:6778-6791(2000)
Lei Q.Y., Zhang H., Zhao B., Zha Z.Y. Mol. Cell. Biol. 28:2426-2436(2008)
Varelas X., Sakuma R., Samavarchi-Tehrani P., Peerani R. Nat. Cell Biol. 10:837-848(2008)
Di Palma T., D'Andrea B., Liguori G.L., Liguoro A. Exp. Cell Res. 315:162-175(2009)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 49kd
Western blotting: 1:500~1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of WWTR1 using anti-WWTR1 antibody (A02672).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WWTR1 antigen affinity purified polyclonal antibody (Catalog # A02672) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WWTR1.

Gene/Protein Information For WWTR1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ27004; FLJ45718; TAZ; TAZDKFZp586I1419; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; WWTR1 WWTR1 TAZ WW domain containing transcription regulator 1 WW domain-containing transcription regulator protein 1|transcriptional co-activator with PDZ-binding motif

For more info on WWTR1, check out the WWTR1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for WWTR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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