Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody

Product Name

Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody

View all Involucrin Antibodies

SKU/Catalog Number

M02628

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody (Catalog # M02628). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Gorilla, Owl monkey, Pig, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02628)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM259

Isotype

IgG1, kappa

Immunogen

Purified involucrin from human keratinocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02628 is reactive to IVL in Dog, Human, Pig, Gorilla, Owl monkey

Applications

M02628 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

68.479kDa

Background of Involucrin

This monoclonal antibody recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, This monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Click image to see more details

Formalin-fixed, paraffin-embedded human Skin stained with Anti-Involucrin Mouse Monoclonal Antibody (SPM259).

Gene/Protein Information For IVL (Source: Uniprot.org, NCBI)

Alternative Names

involucrin IVL involucrin involucrin

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