Product Info Summary
SKU: | A04810-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Tenascin-R/TNR Antibody Picoband™
View all Tenascin R Antibodies
SKU/Catalog Number
A04810-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tenascin-R/TNR Antibody Picoband™ catalog # A04810-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tenascin-R/TNR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04810-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human Tenascin-R/TNR recombinant protein (Position: E60-R1007). Human TNR shares 94.2% and 93.6% amino acid (aa) sequence identity with mouse and rat TNR, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04810-1 is reactive to TNR in Human, Mouse, Rat
Applications
A04810-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
180 kDa
Calculated molecular weight
149.562kDa
Background of Tenascin R
Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R(TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin(TN) gene family, which includes at least 3 genes in mammals: TNC(or hexabrachion), TNX(TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Tenascin-R/TNR using anti-Tenascin-R/TNR antibody (A04810-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tenascin-R/TNR antigen affinity purified polyclonal antibody (Catalog # A04810-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tenascin-R/TNR at approximately 180 kDa. The expected band size for Tenascin-R/TNR is at 150 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNR (Source: Uniprot.org, NCBI)
Gene Name
TNR
Full Name
Tenascin-R
Weight
149.562kDa
Superfamily
tenascin family
Alternative Names
Janusin; MGC149328; Restrictin; tenascin R (restrictin, janusin); Tenascin R; tenascin-R; TNR; TN-R TNR TN-R tenascin R tenascin-R|janusin|restrictin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNR, check out the TNR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tenascin-R/TNR Antibody Picoband™ (A04810-1)
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No publications found for A04810-1
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