Anti-TF3B BRF1 Antibody

Product Name

Anti-TF3B BRF1 Antibody

View all BRF1 Antibodies

SKU/Catalog Number

A30544

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TF3B BRF1 Antibody (Catalog# A30544). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TF3B BRF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30544)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human TF3B. Immunogen sequence location: 231-280

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30544 is reactive to BRF1 in Human, Mouse

Applications

A30544 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

73.84kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western blot analysis of lysates from HeLa and Jurkat cells, using TF3B Antibody. The lane on the right is blocked with the synthesized peptide.

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.

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Immunofluorescence analysis of MCF7 cells, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.

Gene/Protein Information For BRF1 (Source: Uniprot.org, NCBI)

Alternative Names

B-related factor 1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factorIIIB (S. cerevisiae); BRF-1; BRFFLJ42674; general transcription factor IIIB, 90kD subunit; GTF3BTAFIII90; hBRFMGC105048; hTFIIIB90; TAF3B2; TAF3CB - related factor 1; TATA box binding protein (TBP)-associated factor 3C; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3Bsubunit 2; TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; TBP - associated factor, RNA polymerase III BRF1 BRF, BRF-1, CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF BRF1 RNA polymerase III transcription initiation factor subunit transcription factor IIIB 90 kDa subunit|B - related factor 1|BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB|BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit|TATA box binding protein (TBP)-associated factor 3C|TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2|TBP - associated factor, RNA polymerase III, 90kD|epididymis secretory sperm binding protein Li 76p|general transcription factor IIIB, 90kD subunit

For more info on BRF1, check out the BRF1 Infographic

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In this infographic, you will see the following information for BRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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