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Product Info Summary
| SKU: | M01818-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-THY1/Cd90 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01818-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-THY1/Cd90 Rabbit Monoclonal Antibody catalog # M01818-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-THY1/Cd90 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01818-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human THY1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01818-1 is reactive to THY1 in Human
Applications
M01818-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
17.935kDa
Background of THY1
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of CD90 expression in human fetal brain lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using CD90 Antibody.
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Immunofluorescent analysis of U87-MG cells, using CD90 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For THY1 (Source: Uniprot.org, NCBI)
Gene Name
THY1
Full Name
Thy-1 membrane glycoprotein
Weight
17.935kDa
Alternative Names
CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1 THY1 CD90, CDw90 Thy-1 cell surface antigen thy-1 membrane glycoprotein|Thy-1 T-cell antigen|thy-1 antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THY1, check out the THY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-THY1/Cd90 Rabbit Monoclonal Antibody (M01818-1)
Hello CJ!
M01818-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy
Li F, Xu Y, Xu X, Xu B, Zhao J, Zhang X. Mol Med Rep. 2014 Jul;10(1):322-8. Doi: 10.3892/Mmr.2014.2220. Epub 2014 May 8. Fms-Related Tyrosine Kinase??3 Ligand Promotes Proliferation Of Placenta Amnion And Chorion Mesenchymal Stem Cells In??vitro.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-THY1/Cd90 Rabbit Monoclonal Antibody
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-THY1/Cd90 Rabbit Monoclonal antibody supposed to stain brain positively?
K. Lewis
Verified customer
Asked: 2020-01-16
Answer
From literature brain does express THY1. From Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-01-16
Question
We ordered your anti-THY1/Cd90 Rabbit Monoclonal antibody for WB on eye in the past. I am using human, and We want to use the antibody for IF next. you antibody examining eye as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Z. Patel
Verified customer
Asked: 2019-10-14
Answer
I took a look at the website and datasheets of our anti-THY1/Cd90 Rabbit Monoclonal antibody and it seems that M01818-1 has been validated on human in both WB and IF. Thus M01818-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-14
Question
Our team were satisfied with the WB result of your anti-THY1/Cd90 Rabbit Monoclonal antibody. However we have been able to see positive staining in amygdala cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?
S. Singh
Verified customer
Asked: 2015-08-14
Answer
According to literature, amygdala does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2015-08-14
Question
We are currently using anti-THY1/Cd90 Rabbit Monoclonal antibody M01818-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
C. Johnson
Verified customer
Asked: 2015-04-23
Answer
The anti-THY1/Cd90 Rabbit Monoclonal antibody (M01818-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-23
