Anti-TIA1 (T-Cell-Restricted Intracellular Antigen-1) Monoclonal Antibody

Product Name

Anti-TIA1 (T-Cell-Restricted Intracellular Antigen-1) Monoclonal Antibody

View all TIA1 Antibodies

SKU/Catalog Number

M02763

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TIA1 (T-Cell-Restricted Intracellular Antigen-1) Monoclonal Antibody (Catalog # M02763). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TIA1 (T-Cell-Restricted Intracellular Antigen-1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02763)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: TIA1/1313

Isotype

IgG2b, kappa

Immunogen

Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02763 is reactive to TIA1 in Human

Applications

M02763 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

42.963kDa

Background of TIA1

TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15kDa protein thought to be derived from the carboxyl terminus of the 40kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Western Blot of HepG2, PC3 and HeLa cell lysates with Anti-TIA1 Monoclonal Antibody (TIA1/1313).

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Immunofluorescence analysis of PFA-fixed HeLa cells using Anti-TIA1 Mouse Monoclonal Antibody (TIA1/1313) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.

Gene/Protein Information For TIA1 (Source: Uniprot.org, NCBI)

Alternative Names

cytotoxic granule-associated RNA-binding protein; nucleolysin TIA-1 isoform p40; p40-TIA-1 (containing p15-TIA-1); p40-TIA-1; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA1 cytotoxic granule-associated RNA binding protein; TIA1 cytotoxic granule-associated RNA-binding protein; TIA-1 TIA1 ALS26, TIA-1, WDM TIA1 cytotoxic granule associated RNA binding protein nucleolysin TIA-1 isoform p40|nucleolysin TIA-1|T-cell-restricted intracellular antigen-1|p40-TIA-1 (containing p15-TIA-1)

For more info on TIA1, check out the TIA1 Infographic

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In this infographic, you will see the following information for TIA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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