Anti-TMED5/p28 Antibody Picoband™

Product Name

Anti-TMED5/p28 Antibody Picoband™

View all TMED5 Antibodies

SKU/Catalog Number

A12319-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMED5/p28 Antibody Picoband™ catalog # A12319-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TMED5/p28 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12319-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TMED5/p28 recombinant protein (Position: D33-K164).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12319-1 is reactive to TMED5 in Human, Mouse, Rat

Applications

A12319-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

26.005kDa

Background of TMED5

Transmembrane emp24 domain-containing protein 5 is a protein that in humans is encoded by the TMED5 gene. TMED5 is a 229 amino acid single-pass type I membrane protein that belongs to the EMP24/GP25L family and contains one GOLD domain. The gene that encodes TMED5 contains nearly 31,000 bases and maps to human chromosome 1p22.1. As the largest human chromosome, chromosome 1 spans about 260 million base pairs and makes up approximately 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.

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Figure 2. IF analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1).
TMED5/p28 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMED5/p28 Antibody (A12319-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For TMED5 (Source: Uniprot.org, NCBI)

Alternative Names

CGI-100; DKFZp781B2248; transmembrane emp24 domain-containing protein 5; transmembrane emp24 protein transport domain containing 5 TMED5 CGI-100, p24g2, p28 transmembrane p24 trafficking protein 5 transmembrane emp24 domain-containing protein 5|p24 family protein gamma-2|p24gamma2|transmembrane emp24 protein transport domain containing 5

For more info on TMED5, check out the TMED5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TMED5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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