Anti-Toll-like receptor 3 TLR3 Antibody

Product Name

Anti-Toll-like receptor 3 TLR3 Antibody

View all TLR3 Antibodies

SKU/Catalog Number

A00197-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Toll-like receptor 3 TLR3 Antibody (Catalog # A00197-2). Tested in ELISA, WB, ICC, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

TLR3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Toll-like receptor 3 TLR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00197-2)

Host

Rabbit

Contents

TLR3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TLR3 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3. The immunogen is located within amino acids 780 - 830 of TLR3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.

Reactive Species

A00197-2 is reactive to TLR3 in Human, Mouse

Applications

A00197-2 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

103.829kDa

Background of TLR3

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL and immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL.

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Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 μg/mL.

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Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 μg/mL.

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Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 μg/ml.

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Immunohistochemistry of TLR3 in mouse spleen tissue with TLR3 antibody at 2 μg/ml.

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Immunofluorescence of TLR3 in mouse spleen tissue with TLR3 antibody at 20 μg/ml.

Green: TLR3 Antibody (A00197-2)
Blue: DAPI staining

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Immunofluorescence of TLR3 in human spleen tissue with TLR3 antibody at 20 μg/ml.

Green: TLR3 Antibody (A00197-2)
Blue: DAPI staining

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Western blot analysis of TLR3 expression in mouse spleen tissue lysate with TLR3 antibody at (A) 0.5 and (B) 1 μg/ml.

Gene/Protein Information For TLR3 (Source: Uniprot.org, NCBI)

Alternative Names

CD283 antigen; CD283; TLR3; toll-like receptor 3 TLR3 CD283, IIAE2 toll like receptor 3 toll-like receptor 3

For more info on TLR3, check out the TLR3 Infographic

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In this infographic, you will see the following information for TLR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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