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Product Info Summary
| SKU: | A01541-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Toll-like receptor 8 TLR8 Antibody
SKU/Catalog Number
A01541-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody (Catalog # A01541-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TLR8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Toll-like receptor 8 TLR8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01541-1)
Host
Rabbit
Contents
TLR8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TLR8 antibody was raised against a peptide corresponding to 16 amino acids near the middle of human TLR8. The immunogen is located within amino acids 790 - 840 of TLR8.
Reactive Species
A01541-1 is reactive to TLR8 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
119.8 kDa
Background of TLR8
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Like TLR7, TLR8 is localized to endosomal or lysosomal compartments and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA. Human but not murine TLR8 confers responsiveness to the antiviral compound R-848.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01541-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunocytochemistry of TLR8 in Daudi cells with TLR8 antibody at 2 μg/mL.
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Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 μg/mL.
Specific Publications For Anti-Toll-like receptor 8 TLR8 Antibody (A01541-1)
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