Anti-TPX2 Purified Monoclonal Antibody

Product Name

Anti-TPX2 Purified Monoclonal Antibody

View all TPX2 Antibodies

SKU/Catalog Number

M01610

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TPX2 Purified Monoclonal Antibody (Catalog# M01610). Tested in IP, WB, IHC-P, ICC, ELISA application(s). This antibody reacts with Rat, Mouse, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-TPX2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01610)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

TPX2-01

Isotype

Mouse IgG1

Immunogen

Human TPX2. The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01610 is reactive to TPX2 in Human, Mouse, Rat

Applications

M01610 is guaranteed for ELISA, IP, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

85.653kDa

Background of TPX2

TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of TPX2 using Anti-TPX2 Purified Monoclonal Antibody (M01610).

Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase

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Figure 2. IP validation of TPX2 using Anti-TPX2 Purified Monoclonal Antibody (M01610).

Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract

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Figure 3. Western blotting validation for Anti-TPX2 Purified Monoclonal Antibody M01610

Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines

Gene/Protein Information For TPX2 (Source: Uniprot.org, NCBI)

Alternative Names

C20orf1HCTP4; C20orf2; chromosome 20 open reading frame 1; Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL2; DIL-2FLS353; HCA519; Hepatocellular carcinoma-associated antigen 519; P100; p100GD:C20orf1; preferentially expressed in colorectal cancer; Protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; targeting protein for Xklp2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog (Xenopus laevis) TPX2 C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100 TPX2 microtubule nucleation factor targeting protein for Xklp2|TPX2, microtubule-associated protein homolog|TPX2, microtubule-associated, homolog|differentially expressed in cancerous and non-cancerous lung cells 2|differentially expressed in lung cells|hepatocellular carcinoma-associated antigen 519|hepatocellular carcinoma-associated antigen 90|preferentially expressed in colorectal cancer|protein fls353|restricted expression proliferation associated protein 100

For more info on TPX2, check out the TPX2 Infographic

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In this infographic, you will see the following information for TPX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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