Click image to see more details
Product Info Summary
| SKU: | M00185 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRAF6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00185
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRAF6 Rabbit Monoclonal Antibody catalog # M00185. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00185)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRAF6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00185 is reactive to TRAF6 in Human, Mouse, Rat
Applications
M00185 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
59.573kDa
Background of TRAF-6
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of TRAF6 expression in Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TRAF6 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TRAF6 (Source: Uniprot.org, NCBI)
Gene Name
TRAF6
Full Name
TNF receptor-associated factor 6
Weight
59.573kDa
Superfamily
TNF receptor-associated factor family
Alternative Names
E3 ubiquitin-protein ligase TRAF6; EC 6.3.2.-; Interleukin-1 signal transducer; RING finger protein 85; RNF85MGC:3310; TNF receptor-associated factor 6; TRAF6; TRAF-6 TRAF6 MGC:3310, RNF85 TNF receptor associated factor 6 TNF receptor-associated factor 6|E3 ubiquitin-protein ligase TRAF6|RING finger protein 85|RING-type E3 ubiquitin transferase TRAF6|TNF receptor-associated factor 6, E3 ubiquitin protein ligase|interleukin-1 signal transducer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF6, check out the TRAF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF6 Rabbit Monoclonal Antibody (M00185)
Hello CJ!
M00185 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00185 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:500
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Yuan Z, Zhang J, Yang C. Evid Based Complement Alternat Med. 2014;2014:620359. Doi: 10.1155/2014/620359. Epub 2014 Aug 17. Ligusticum Wallichii Extract Inhibited The Expression Of Il-1?? After Ami In Rats.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-TRAF6 Rabbit Monoclonal Antibody
Question
Would M00185 anti-TRAF6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, M00185 anti-TRAF6 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-TRAF6 Rabbit Monoclonal antibody M00185 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-05
Question
Does anti-TRAF6 Rabbit Monoclonal antibody M00185 work for IF with secondary oocyte?
N. Evans
Verified customer
Asked: 2016-03-10
Answer
According to the expression profile of secondary oocyte, TRAF6 is highly expressed in secondary oocyte. So, it is likely that anti-TRAF6 Rabbit Monoclonal antibody M00185 will work for IF with secondary oocyte.
Boster Scientific Support
Answered: 2016-03-10
