Product Info Summary
SKU: | A00662-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Transcription factor EB TFEB Antibody
SKU/Catalog Number
A00662-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Transcription factor EB TFEB Antibody catalog # A00662-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor EB TFEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00662-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human TFEB
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00662-1 is reactive to TFEB in Human, Mouse
Applications
A00662-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.865kDa
Background of TFEB
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Transcription factor EB TFEB Antibody A00662-1
Western Blot (WB) analysis of Mouse Lung Mouse Muscle cells using TFEB polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-Transcription factor EB TFEB Antibody A00662-1
Western Blot (WB) analysis of Mouse Kidney lysis using TFEB antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TFEB (Source: Uniprot.org, NCBI)
Gene Name
TFEB
Full Name
Transcription factor EB
Weight
52.865kDa
Superfamily
MiT/TFE family
Alternative Names
ALPHATFEB; BHLHE35; Class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; TCFEBbHLHe35; TFEB; transcription factor EB TFEB ALPHATFEB, BHLHE35, TCFEB transcription factor EB transcription factor EB|T-cell transcription factor EB|class E basic helix-loop-helix protein 35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFEB, check out the TFEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor EB TFEB Antibody (A00662-1)
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No publications found for A00662-1
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