Anti-Transcription factor HES-5 HES5 Antibody

Product Name

Anti-Transcription factor HES-5 HES5 Antibody

View all Hes5 Antibodies

SKU/Catalog Number

A05384

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transcription factor HES-5 HES5 Antibody (Catalog # A05384). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

HES5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Transcription factor HES-5 HES5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05384)

Host

Rabbit

Contents

HES5 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.

Reactive Species

A05384 is reactive to Hes5 in Human, Mouse, Rat

Applications

A05384 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

18.425kDa

Background of Hes5

HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.

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Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.

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Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μg/mL.

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Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μg/mL.

Green: HES5 Antibody (A05384)
Blue: DAPI staining

Gene/Protein Information For Hes5 (Source: Uniprot.org, NCBI)

Alternative Names

BHLHB38; bHLHb38Class B basic helix-loop-helix protein 38; hairy and enhancer of split 5 (Drosophila); Hairy and enhancer of split 5; transcription factor HES-5 Hes5|bHLHb3, bHLHb38|hes family bHLH transcription factor 5|transcription factor HES-5|hairy and enhancer of split 5

For more info on Hes5, check out the Hes5 Infographic

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In this infographic, you will see the following information for Hes5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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