Anti-TREML1 Antibody Picoband™

Product Name

Anti-TREML1 Antibody Picoband™

View all TREML1/TLT-1 Antibodies

SKU/Catalog Number

A07682-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TREML1 Antibody Picoband™ catalog # A07682-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TREML1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07682-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TREML1 recombinant protein (Position: Q16-P162). Human TREML1 shares 69.3% amino acid (aa) sequence identity with mouse TREML1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07682-2 is reactive to Treml1 in Human

Applications

A07682-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

33.522kDa

Background of TREML1/TLT-1

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TREML1 using anti-TREML1 antibody (A07682-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREML1 antigen affinity purified polyclonal antibody (Catalog # A07682-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREML1 at approximately 33KD. The expected band size for TREML1 is at 33KD.

Gene/Protein Information For Treml1 (Source: Uniprot.org, NCBI)

Alternative Names

dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1 Treml1|5430401J17Rik, TLT-, TLT-1|triggering receptor expressed on myeloid cells-like 1|trem-like transcript 1 protein|triggering receptor expressed on myeloid cells-like 1c|triggering receptor expressed on myeloid cells-like 1d|triggering receptor expressed on myeloid cells-like 1e|triggering receptor expressed on myeloid cells-like protein 1

For more info on Treml1, check out the Treml1 Infographic

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In this infographic, you will see the following information for Treml1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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