Human TNF-alpha Recombinant Protein

TNF-alpha protein,

Human TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 77-233aa. Application(s): SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Product Info Summary

SKU: PROTP01375
Size: Starting from 10ug
Source: Baculovirus
Application: SDS-PAGE, Bioactivity

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Product Name

Human TNF-alpha Recombinant Protein

View all TNF-alpha recombinant proteins

SKU/Catalog Number

PROTP01375

Size

Starting from 10ug

Tag

His-Tag

Description

Human TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 77-233aa. Application(s): SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Human TNF-alpha Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01375)

Form

Liquid

Formulation

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Concentration

1mg/ml (determined by absorbance at 280nm)

Purity

> 90% by SDS-PAGE

Predicted MW

25.644kDa

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence

Human, P01375, 77-233aa; VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH

Bioactivity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/ml.

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Validation Images & Assay Conditions

Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)

Gene Name

TNF

Full Name

Tumor necrosis factor

Weight

25.644kDa

Superfamily

Tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on TNF, check out the TNF Infographic

TNF infographic

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In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $250

SKU:PROTP01375

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