Anti-TRPC6 Antibody Picoband™

Product Name

Anti-TRPC6 Antibody Picoband™

View all TRPC6 Antibodies

SKU/Catalog Number

A00625

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRPC6 Antibody Picoband™ catalog # A00625. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRPC6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00625)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TRPC6 recombinant protein (Position: K771-E875). Human TRPC6 shares 82.9% amino acid (aa) sequence identity with mouse TRPC6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00625 is reactive to TRPC6 in Human, Mouse, Rat

Applications

A00625 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

106 kDa

Calculated molecular weight

106.326kDa

Background of TRPC6

Transient receptor potential cation channel, subfamily C, member 6, also known as TRPC6, is a human gene encoding a protein of the same name. The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysate,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # A00625) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.

Gene/Protein Information For TRPC6 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ11098; FSGS2; short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; TRP6FLJ14863; TrpC6 TRPC6 FSGS2, TRP6 transient receptor potential cation channel subfamily C member 6 short transient receptor potential channel 6|TRP-6|focal segmental glomerulosclerosis 2|transient receptor protein 6

For more info on TRPC6, check out the TRPC6 Infographic

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In this infographic, you will see the following information for TRPC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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