Product Info Summary
SKU: | A12386-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TSEN34 Antibody Picoband™
SKU/Catalog Number
A12386-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSEN34 Antibody Picoband™ catalog # A12386-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TSEN34 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12386-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TSEN34 recombinant protein (Position: M1-Q310).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12386-1 is reactive to Tsen34 in Human, Mouse, Rat
Applications
A12386-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
34.196kDa
Background of Tsen34
tRNA-splicing endonuclease subunit Sen34 is an enzyme that in humans is encoded by the TSEN34 gene. This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TSEN34 using anti-TSEN34 antibody (A12386-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN34 antigen affinity purified polyclonal antibody (Catalog # A12386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN34 at approximately 50 kDa. The expected band size for TSEN34 is at 34 kDa.
Click image to see more details
Figure 2. IF analysis of TSEN34 and Tubulin beta using anti-TSEN34 antibody (A12386-1) and anti-Tubulin beta antibody (M05613-4).
TSEN34 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSEN34 Antibody (A12386-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Tsen34 (Source: Uniprot.org, NCBI)
Gene Name
Tsen34
Full Name
tRNA-splicing endonuclease subunit Sen34
Weight
34.196kDa
Superfamily
tRNA-intron endonuclease family
Alternative Names
EC 3.1.27.9; hsSen34; leukocyte receptor cluster (LRC) member 5; Leukocyte receptor cluster member 5; PCH2C; SEN34LENG5SEN34L; tRNA splicing endonuclease 34 homolog (S. cerevisiae); tRNA splicing endonuclease 34 homolog (SEN34, S. cerevisiae); tRNA-intron endonuclease Sen34; tRNA-splicing endonuclease subunit Sen34
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tsen34, check out the Tsen34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tsen34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSEN34 Antibody Picoband™ (A12386-1)
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No publications found for A12386-1
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