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Product Info Summary
| SKU: | RP1078 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TSG101 Antibody
SKU/Catalog Number
RP1078
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSG101 Antibody catalog # RP1078. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSG101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1078)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TSG101, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1078 is reactive to TSG101 in Human, Rat
Applications
RP1078 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
43.944kDa
Background of TSG101
TSG101, known as Tumor susceptibility gene 101, is mapped to 11p15. The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. And the protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
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Anti-TSG101 antibody, RP1078, Western blotting
All lanes: Anti TSG101 (RP1078) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
Protein Target Info & Infographic
Gene/Protein Information For TSG101 (Source: Uniprot.org, NCBI)
Gene Name
TSG101
Full Name
Tumor susceptibility gene 101 protein
Weight
43.944kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101 protein; tumor susceptibility gene 101; tumor susceptibility protein; VPS23 TSG101 TSG10, VPS23 tumor susceptibility 101 tumor susceptibility gene 101 protein|ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSG101, check out the TSG101 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSG101: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSG101 Antibody (RP1078)
Hello CJ!
RP1078 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tian F,Tang P,Sun Z,Zhang R,Zhu D,He J,Liao J,Wan Q,Shen J.miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression.J Diabetes Res.2020 Mar 19;2020:6894684.doi:10.1155/2020/6894684.PMID:32258168;PMCID:PMC7106924.
Species: Mouse
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
RP1078 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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4 Customer Q&As for Anti-TSG101 Antibody
Question
Our team were happy with the WB result of your anti-TSG101 antibody. However we have observed positive staining in oocyte cytoplasm. using this antibody. Is that expected? Could you tell me where is TSG101 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
From what I have seen in literature, oocyte does express TSG101. Generally TSG101 expresses in cytoplasm. Regarding which tissues have TSG101 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Placenta, Pubmed ID: 9019400
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-TSG101 antibody RP1078 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The anti-TSG101 antibody (RP1078) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-16
Question
We have seen staining in rat eye. Do you have any suggestions? Is anti-TSG101 antibody supposed to stain eye positively?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
Based on literature eye does express TSG101. Based on Uniprot.org, TSG101 is expressed in oocyte, placenta, eye, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TSG101 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Placenta, Pubmed ID: 9019400
Boster Scientific Support
Answered: 2018-12-19
Question
I was wanting to use using your anti-TSG101 antibody for viral life cycle studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
Thank you for your inquiry. This RP1078 anti-TSG101 antibody is tested on rat brain tissue, cardiac muscle tissue, tissue lysate, hela whole cell lysate, smmc whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-28
