Product Info Summary
SKU: | M01233 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TSG101 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01233
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TSG101 Rabbit Monoclonal Antibody catalog # M01233. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSG101 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01233)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TSG101
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01233 is reactive to TSG101 in Human, Mouse, Rat
Applications
M01233 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
43.944kDa
Background of TSG101
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (M01233).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified monoclonal antibody (Catalog # M01233) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:1000 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:500 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse kidney, using the Antibody at 1:1000 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:500 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human tongue cancer, using the Antibody at 1:250 dilution.
Protein Target Info & Infographic
Gene/Protein Information For TSG101 (Source: Uniprot.org, NCBI)
Gene Name
TSG101
Full Name
Tumor susceptibility gene 101 protein
Weight
43.944kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101 protein; tumor susceptibility gene 101; tumor susceptibility protein; VPS23 TSG101 TSG10, VPS23 tumor susceptibility 101 tumor susceptibility gene 101 protein|ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSG101, check out the TSG101 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSG101: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSG101 Rabbit Monoclonal Antibody (M01233)
Hello CJ!
M01233 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
M01233 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-TSG101 Rabbit Monoclonal Antibody
Question
Can you help my question with product M01233, anti-TSG101 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01233 anti-TSG101 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
Our lab want to know about using your anti-TSG101 Rabbit Monoclonal antibody for protein monoubiquitination studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We appreciate your inquiry. This M01233 anti-TSG101 Rabbit Monoclonal antibody is validated on jurkat cell lysate, hela cell lysate, k562 cell lysate, a431 cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-30
Question
Will M01233 anti-TSG101 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It shows on the product datasheet, M01233 anti-TSG101 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
Is this M01233 anti-TSG101 Rabbit Monoclonal antibody reactive to the isotypes of TSG101?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of M01233 anti-TSG101 Rabbit Monoclonal antibody is A synthesized peptide derived from human TSG101. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
Our lab were well pleased with the WB result of your anti-TSG101 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is TSG101 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to literature, cervix carcinoma erythroleukemia does express TSG101. Generally TSG101 expresses in cytoplasm. Regarding which tissues have TSG101 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Placenta, Pubmed ID: 9019400
Boster Scientific Support
Answered: 2019-12-24
Question
We need to test anti-TSG101 Rabbit Monoclonal antibody M01233 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-TSG101 Rabbit Monoclonal antibody M01233 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The products we sell, including anti-TSG101 Rabbit Monoclonal antibody M01233, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-09
Question
I was wanting to use your anti-TSG101 Rabbit Monoclonal antibody for IF for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, M01233 anti-TSG101 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-TSG101 Rabbit Monoclonal antibody M01233 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-18
Question
We purchased anti-TSG101 Rabbit Monoclonal antibody for IF on oocyte in a previous project. I am using rat, and We want to use the antibody for WB next. I would like examining oocyte as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
I looked at the website and datasheets of our anti-TSG101 Rabbit Monoclonal antibody and it seems that M01233 has been validated on rat in both IF and WB. Thus M01233 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-23
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TSG101 Rabbit Monoclonal antibody M01233. Please let me know if you require anything else.
P. Carter
Verified customer
Asked: 2018-12-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-05
Question
Will anti-TSG101 Rabbit Monoclonal antibody M01233 work for IF with cervix carcinoma erythroleukemia?
J. Wu
Verified customer
Asked: 2018-11-08
Answer
According to the expression profile of cervix carcinoma erythroleukemia, TSG101 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-TSG101 Rabbit Monoclonal antibody M01233 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-11-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TSG101 Rabbit Monoclonal antibody M01233. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-30
Question
Is a blocking peptide available for product anti-TSG101 Rabbit Monoclonal antibody (M01233)?
C. Jones
Verified customer
Asked: 2017-05-19
Answer
We do provide the blocking peptide for product anti-TSG101 Rabbit Monoclonal antibody (M01233). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-19
Question
We are currently using anti-TSG101 Rabbit Monoclonal antibody M01233 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Z. Jones
Verified customer
Asked: 2016-12-13
Answer
The anti-TSG101 Rabbit Monoclonal antibody (M01233) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-13
Question
We have observed staining in human eye. Are there any suggestions? Is anti-TSG101 Rabbit Monoclonal antibody supposed to stain eye positively?
C. Carter
Verified customer
Asked: 2013-11-08
Answer
According to literature eye does express TSG101. According to Uniprot.org, TSG101 is expressed in oocyte, placenta, eye, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TSG101 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Placenta, Pubmed ID: 9019400
Boster Scientific Support
Answered: 2013-11-08
Question
Is there a BSA free version of anti-TSG101 Rabbit Monoclonal antibody M01233 available?
G. Zhao
Verified customer
Asked: 2013-01-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TSG101 Rabbit Monoclonal antibody M01233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-18