Product Info Summary
SKU: | A02462-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-UBC13 UBE2N Antibody
View all UBE2N/Ubc13 Antibodies
SKU/Catalog Number
A02462-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-UBC13 UBE2N Antibody (Catalog # A02462-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
UBC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-UBC13 UBE2N Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02462-1)
Host
Rabbit
Contents
UBC13 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13. The immunogen is located within the last 50 amino acids of UBC13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Reactive Species
A02462-1 is reactive to UBE2N in Human, Mouse, Rat
Applications
A02462-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.138kDa
Background of UBE2N/Ubc13
Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway. It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
UBC13 antibody can be used for detection of UBC13 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 μg/mL.
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Immunofluorescence of UBC13 in Mouse Thymus cells with UBC13 antibody at 10 μg/mL.
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Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2.5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For UBE2N (Source: Uniprot.org, NCBI)
Gene Name
UBE2N
Full Name
Ubiquitin-conjugating enzyme E2 N
Weight
17.138kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog UBE2N HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13 ubiquitin conjugating enzyme E2 N ubiquitin-conjugating enzyme E2 N|E2 ubiquitin-conjugating enzyme N|bendless-like ubiquitin conjugating enzyme|epididymis secretory protein Li 71|ubiquitin carrier protein N|ubiquitin conjugating enzyme E2N|ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)|ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)|ubiquitin-protein ligase N|yeast UBC13 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2N, check out the UBE2N Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2N: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBC13 UBE2N Antibody (A02462-1)
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