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Product Info Summary
| SKU: | M07478 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-UBE2M Mouse Monoclonal Antibody [Clone ID: OTI2D9]
View all UBE2M/Ubc12 Antibodies
SKU/Catalog Number
M07478
Size
100 µl
Description
Boster Bio UBE2M mouse monoclonal antibody, clone OTI2D9 (formerly 2D9). Catalog# M07478. Tested in IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-UBE2M Mouse Monoclonal Antibody [Clone ID: OTI2D9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07478)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2D9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human UBE2M (NP_003960) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07478 is reactive to UBE2M in Dog, Human, Monkey, Mouse, Rat
Applications
M07478 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
20.9kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.
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Anti-UBE2M mouse monoclonal antibody (M07478) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
Protein Target Info & Infographic
Gene/Protein Information For UBE2M (Source: Uniprot.org, NCBI)
Gene Name
UBE2M
Full Name
NEDD8-conjugating enzyme Ubc12
Weight
20.9kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12; UBC12NEDD8-conjugating enzyme Ubc12; UbcH12; UBC-RS2; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog UBE2M UBC-RS2, UBC12, hUbc12 ubiquitin conjugating enzyme E2 M NEDD8-conjugating enzyme Ubc12|NEDD8 carrier protein|NEDD8 protein ligase|epididymis secretory sperm binding protein|ubiquitin carrier protein M|ubiquitin conjugating enzyme E2M|ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)|ubiquitin-protein ligase M|yeast UBC12 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2M, check out the UBE2M Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2M: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2M Mouse Monoclonal Antibody [Clone ID: OTI2D9] (M07478)
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