Anti-Ugt1a1 Antibody Picoband™

Product Name

Anti-Ugt1a1 Antibody Picoband™

View all Glucuronosyltransferase 1A1/UGT1A1 Antibodies

SKU/Catalog Number

A01865-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ugt1a1 Antibody Picoband™ catalog # A01865-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ugt1a1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01865-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Ugt1a1 recombinant protein (Position: H57-D248).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01865-2 is reactive to Ugt1a1 in Mouse, Rat

Applications

A01865-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

50433 MW

Background of Glucuronosyltransferase 1A1/UGT1A1

UDP-glucuronosyltransferase 1-1 also known as UGT-1A is an enzyme that in humans is encoded by the UGT1A1 gene. Predicted to enable several functions, including glucuronosyltransferase activity; protein dimerization activity; and retinoic acid binding activity. Predicted to be involved in several processes, including biphenyl catabolic process; cellular glucuronidation; and negative regulation of cellular glucuronidation. Is integral component of plasma membrane. Part of endoplasmic reticulum chaperone complex. Is expressed in alimentary system; nervous system; and urinary system. Human ortholog(s) of this gene implicated in several diseases, including bilirubin metabolic disorder (multiple); cholecystolithiasis; cholelithiasis; female reproductive organ cancer (multiple); and hepatitis B. Orthologous to human UGT1A1 (UDP glucuronosyltransferase family 1 member A1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ugt1a1 using anti-Ugt1a1 antibody (A01865-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ugt1a1 antigen affinity purified polyclonal antibody (Catalog # A01865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ugt1a1 at approximately 55 kDa. The expected band size for Ugt1a1 is at 60 kDa.

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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Ugt1a1 antibody (A01865-2).
Overlay histogram showing ANA-1 cells stained with A01865-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ugt1a1 Antibody (A01865-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Ugt1a1 (Source: Uniprot.org, NCBI)

Alternative Names

bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; Bilirubin-specific UDPGT isozyme 1; GNT1; GNT1EC 2.4.1.17; HUG-BR1; UDP glucuronosyltransferase 1 family, polypeptide A1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDPGT 1-1; UDPGT; UG-BR1; UGT1; UGT1*1; UGT1.1; UGT1-01; UGT1A; UGT-1A; UGT1A1; UGT1AUDPGT 1-1; UGT1BILIQTL1 Ugt1a1|UDPGT 1-1, Udpgt, Ugt1|UDP glucuronosyltransferase family 1 member A1|UDP-glucuronosyltransferase 1A1|B1|UDP glucuronosyltransferase 1 family, polypeptide A1|UDP glycosyltransferase 1 family, polypeptide A1|UDP-glucuronosyltransferase 1 family member 1|UDP-glucuronosyltransferase 1-1|UGT1*1|UGT1-01|UGT1.1

For more info on Ugt1a1, check out the Ugt1a1 Infographic

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In this infographic, you will see the following information for Ugt1a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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