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Product Info Summary
| SKU: | PA2079 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-VE-Cadherin CDH5-Antibody
View all VE-Cadherin Antibodies
SKU/Catalog Number
PA2079
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VE-Cadherin CDH5-Antibody catalog # PA2079. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VE-Cadherin CDH5-Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2079)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VE Cadherin, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2079 is reactive to CDH5 in Human, Mouse, Rat
Applications
PA2079 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
87.528kDa
Background of VE-Cadherin
CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. Kremmidiotis et al. (1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Validation Images & Assay Conditions
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Anti-VE Cadherin antibody, PA2079, Western blotting
All lanes: Anti VE Cadherin (PA2079) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 130KD
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Anti-VE Cadherin antibody, PA2079, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-VE Cadherin antibody, PA2079, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For CDH5 (Source: Uniprot.org, NCBI)
Gene Name
CDH5
Full Name
Cadherin-5
Weight
87.528kDa
Alternative Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin CDH5 7B4, CD144 cadherin 5 cadherin-5|7B4 antigen|VE-cadherin|cadherin 5, type 2 (vascular endothelium)|cadherin 5, type 2, VE-cadherin (vascular epithelium)|cd144 antigen|endothelial-specific cadherin|vascular endothelial cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH5, check out the CDH5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VE-Cadherin CDH5-Antibody (PA2079)
Hello CJ!
PA2079 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage
Chen H,Chen B,Li B,Luo X,Wu H,Zhang C,Liu J,Jiang J,Zhao B. Gastrodin Promotes the Survival of Random-Pattern Skin Flaps via Autophagy Flux Stimulation. Oxid Med Cell Longev. 2021 Jan 9;2021:6611668.doi:10.1155/2021/6611668.PMID:33505583;PMCID:PMC7811417.
Species: Rat
Li J,Chen H,Lou J,Bao G,Wu C,Lou Z,Wang X,Ding J,Li Z,Xiao J,Xu H,Gao W,Zhou K.Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.J Cell Physiol.2020 Oct 12.doi:10.1002/jcp.30102.Epub ahead of print.PMID:33044023.
Species: Mouse
PA2079 usage in article: APP:WB, SAMPLE:FLAP TISSUE, DILUTION:1:1000
Exposure to Concentrated Ambient Fine Particulate Matter Induces Vascular Endothelial Dysfunction via miR-21
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Anti-VE-Cadherin CDH5-Antibody
Question
Is a blocking peptide available for product anti-VE Cadherin/CDH5 antibody (PA2079)?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We do provide the blocking peptide for product anti-VE Cadherin/CDH5 antibody (PA2079). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-26
Question
Would PA2079 anti-VE Cadherin/CDH5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
You can see on the product datasheet, PA2079 anti-VE Cadherin/CDH5 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-26
Question
We are currently using anti-VE Cadherin/CDH5 antibody PA2079 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
B. Huang
Verified customer
Asked: 2019-10-31
Answer
The anti-VE Cadherin/CDH5 antibody (PA2079) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
Is there a BSA free version of anti-VE Cadherin/CDH5 antibody PA2079 available?
D. Parker
Verified customer
Asked: 2019-10-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VE Cadherin/CDH5 antibody PA2079 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
Has PA2079 been tested with WB on mouse tissue samples?
Verified customer
Asked: 2019-04-23
Answer
The test result on mouse samples for WB is not good for the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079). We recommend to use the Anti-VE-Cadherin-5 Cdh5-Antibody Picoband™ (A02632-2) for this instead.
Boster Scientific Support
Answered: 2019-04-24
Question
Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?
Verified customer
Asked: 2019-04-18
Answer
Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.
Boster Scientific Support
Answered: 2019-04-19
Question
Does anti-VE Cadherin/CDH5 antibody PA2079 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
According to the expression profile of placenta, CDH5 is highly expressed in placenta. So, it is likely that anti-VE Cadherin/CDH5 antibody PA2079 will work for IHC with placenta.
Boster Scientific Support
Answered: 2018-08-27
Question
Will anti-VE Cadherin/CDH5 antibody PA2079 work on goat WB with endothelial cell?
J. Zhang
Verified customer
Asked: 2018-03-06
Answer
Our lab technicians have not validated anti-VE Cadherin/CDH5 antibody PA2079 on goat. You can run a BLAST between goat and the immunogen sequence of anti-VE Cadherin/CDH5 antibody PA2079 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat endothelial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-06
Question
I am looking for to test anti-VE Cadherin/CDH5 antibody PA2079 on human placenta for research purposes, then I may be interested in using anti-VE Cadherin/CDH5 antibody PA2079 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
The products we sell, including anti-VE Cadherin/CDH5 antibody PA2079, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-16
