SKU PA1080
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-VEGF/VEGFA Antibody
SKU/Catalog Number PA1080
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-VEGF/VEGFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1080)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1080 1 WB anti vegf antibody.jpg

Anti-VEGF antibody, PA1080, Western blotting
Lane 1: Recombinant Human VEGF Protein 10ng
Lane 2: Recombinant Human VEGF Protein 5ng
Lane 3: Recombinant Human VEGF Protein 2.5ng

/antibody/pa1080 2 IHC anti vegf antibody.jpg

Anti-VEGF antibody, PA1080, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P15692
Gene Name VEGFA
Protein Name Vascular endothelial growth factor A
Tissue Specificity Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .
Alternative Names Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;VEGFA;VEGF;
Subcellular Localization Secreted . VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
Molecular Weight 27042 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. .
Research Areas Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways And Processes, Response To Hypoxia, Signal Transduction

*You can search these to find other products in these research areas.
Background VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor(VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor(VEGF) stimulates neurogenesis in vitro and in vivo.

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Order Product (PA1080)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

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Troubleshooting

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Publications

Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis
Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
Proper autophagy is indispensable for angiogenesis during chick embryo development
VEGFR-2 conformational switch in response to ligand binding
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head
Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head
The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice
Insufficient radiofrequency ablation promotes human hepatoma SMMC7721 cell proliferation by stimulating vascular endothelial growth factor overexpression
Ectopic expression of AP-2? transcription factor suppresses glioma progression
microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma
Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer
HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head
Roxarsone induces angiogenesis via PI3K/Akt signaling
Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1
Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair
Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
Correlation and Significance of Urinary Soluble Fas and Vascular Endothelial Growth Factor in Bladder Urothelial Cancer
Factors influencing improvement of visual field after trans-sphenoidal resection of pituitary macroadenomas: a retrospective cohort study
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells
Ning D, Zhang WK, Tian H, Li XJ, Liu M, Li YS, Tang HB. J Ophthalmol. 2017;2017:9760501. doi: 10.1155/2017/9760501. Epub 2017 Oct 12. The Involvement of β-Catenin/COX-2/VEGF Axis in NMDA-Caused Retinopathy
Ma X, Li Z, Li T, Zhu L, Li Z, Tian N. Am J Transl Res. 2017 Nov 15;9(11):5012-5021. eCollection 2017. Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glio...
Yan-Ping D, Xiao-Qin G, Xiao Ping M, Ying Quan Y. Biomed Res Int. 2017;2017:2597256. doi: 10.1155/2017/2597256. Epub 2017 Jul 16. Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats
Qin JH, Ke ZY, Zhou Q, Wang L, Liang Y, Wang YM, Yang T, Gao X, Ye J, Kumar R, Wang RA,. Med Sci Monit. 2017 Aug 15;23:3932-3941. Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice
Li P, Chen H, Chen S, Mo X, Li T, Xiao B, Yu R, Guo J. Br J Cancer. 2017 Feb 28;116(5):626-633. doi: 10.1038/bjc.2016.451. Epub 2017 Jan 12 Circular RNA 0000096 affects cell growth and migration in gastric cancer
Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts
Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010
Sun J, Wang S, Wei Yh. J Ethnopharmacol. 2011 Oct 11;137(3):1498-503. Doi: 10.1016/J.Jep.2011.08.026. Epub 2011 Aug 22. Reproductive Toxicity Of Rhizoma Sparganii (Sparganium Stoloniferum Buch.-Ham.) In Mice: Mechanisms Of Anti-Angiogenesis And An...
Zhu L, Wei W, Zheng Yq, Jia Xy. Inflamm Res. 2005 May;54(5):211-20. Effects And Mechanisms Of Total Glucosides Of Paeony On Joint Damage In Rat Collagen-Induced Arthritis.
Liu S, Liu T, Chen J, Maitz M, Chen C, Huang N. J Biomed Mater Res A. 2013 Apr;101(4):1144-57. Doi: 10.1002/Jbm.A.34392. Epub 2012 Oct 9. Influence Of A Layer-By-Layer-Assembled Multilayer Of Anti-Cd34 Antibody, Vascular Endothelial Growth Factor,...
Su B, Xiang B, Wang L, Cao L, Xiao L, Li X, Li X, Wu M, Li G. J Cell Biochem. 2010 Jan 1;109(1):173-83. Doi: 10.1002/Jcb.22395. Profiling And Comparing Transcription Factors Activated In Non-Metastatic And Metastatic Nasopharyngeal Carcinoma Cells.
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Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...
Zhang B, Zhang Y, Yao G, Gao J, Yang B, Zhao Y, Rao Z, Gao J. Clinics (Sao Paulo). 2012 Aug;67(8):901-6. M2-Polarized Macrophages Promote Metastatic Behavior Of Lewis Lung Carcinoma Cells By Inducing Vascular Endothelial Growth Factor-C Expression.
Xuebing P, Tinchiu L, Enlan X, Jing L, Xiaowu H. Eur J Obstet Gynecol Reprod Biol. 2011 Nov;159(1):198-203. Doi: 10.1016/J.Ejogrb.2011.06.036. Epub 2011 Jul 2. Is Endometrial Polyp Formation Associated With Increased Expression Of Vascular Endothe...
Jin M, Zhang Y, Pan L, Dou R, Nussenblatt Rb, Wei L. J Transl Med. 2012 Jun 7;10:118. Doi: 10.1186/1479-5876-10-118. The Chinese Medicine Formula Hb01 Reduces Choroidal Neovascularization By Regulating The Expression Of Vascular Endothelial Growth...
Li X, Wang H, Lu X, Di B. Exp Ther Med. 2010 Nov;1(6):947-953. Epub 2010 Sep 29. Silencing Stat3 With Short Hairpin Rna Enhances Radiosensitivity Of Human Laryngeal Squamous Cell Carcinoma Xenografts In Vivo.
Zhang S, Yu H, Zhang L. Oncol Lett. 2010 May;1(3):453-456. Epub 2010 May 1. Role Of Vascular Endothelial Growth Factor Receptor-3/Flt-4 In Early-Stage Cervical Cancer.
Tang C, Wu X, Ye L, Xie X, Wang G. Biocell. 2012 Dec;36(3):97-103. Effects Of Rotational Culture On Morphology, Nitric Oxide Production And Cell Cycle Of Endothelial Cells.
Zhao W, Shen H, Yuan F, Li G, Sun Y, Shi Z, Zhang Y, Wang Z. Eur J Histochem. 2009 Jan-Mar;53(1):53-60. Induction Stage-Dependent Expression Of Vascular Endothelial Growth Factor And Aquaporin-1 In Diethylstilbestrol-Treated Rat Pituitary.
Liang Ql, Wang Br, Chen Gq, Li Gh, Xu Yy. Med Oncol. 2010 Dec;27(4):1164-70. Doi: 10.1007/S12032-009-9354-1. Epub 2009 Nov 12. Clinical Significance Of Vascular Endothelial Growth Factor And Connexin43 For Predicting Pancreatic Cancer Clinicopatho...
Zhan Cy, Yuan Tm, Sun Y, Yu Hm. J Matern Fetal Neonatal Med. 2011 Feb;24(2):213-22. Doi: 10.3109/14767051003758895. Epub 2010 May 17. Early Gestational Intrauterine Infection Induces Postnatal Lung Inflammation And Arrests Lung Development In A Ra...
Zhang Y, Zhang H, Chen J, Zhao H, Zeng X, Zhang H, Qing C. Invest New Drugs. 2012 Feb;30(1):8-16. Doi: 10.1007/S10637-010-9501-8. Epub 2010 Aug 14. Antitumor And Antiangiogenic Effects Of Ga-13315, A Gibberellin Derivative.
Zhang L, Ge W, Hu K, Zhang Y, Li C, Xu X, He D, Zhao Z, Zhang J, Jie F, Chen Y, Zheng Y. Mol Biol Rep. 2012 Jan;39(1):89-95. Doi: 10.1007/S11033-011-0713-6. Epub 2011 May 13. Endostar Down-Regulates Hif-1 And Vegf Expression And Enhances The Radio...
Jiang X, Ge H, Zhou C, Chai X, Ren Qs. Lasers Med Sci. 2012 May;27(3):599-606. Doi: 10.1007/S10103-011-0996-9. Epub 2011 Oct 1. The Role Of Vascular Endothelial Growth Factor In Fractional Laser Resurfacing With The Carbon Dioxide Laser.
Guo Q, Jin J, Yuan Jx, Zeifman A, Chen J, Shen B, Huang J. Biochem Biophys Res Commun. 2011 Oct 7;413(4):630-6. Doi: 10.1016/J.Bbrc.2011.09.015. Epub 2011 Sep 12. Vegf, Bcl-2 And Bad Regulated By Angiopoietin-1 In Oleic Acid Induced Acute Lung Inj...
Zhang T, Liu M, Wang C, Lin C, Sun Y, Jin D. Anticancer Res. 2011 Nov;31(11):3859-63. Down-Regulation Of Mir-206 Promotes Proliferation And Invasion Of Laryngeal Cancer By Regulating Vegf Expression.
Zhang W, Yan H. Exp Eye Res. 2012 Dec;105:1-8. Doi: 10.1016/J.Exer.2012.09.014. Epub 2012 Oct 8. Simvastatin Increases Circulating Endothelial Progenitor Cells And Reduces The Formation And Progression Of Diabetic Retinopathy In Rats.
Jia Y, Wu J, Chen L, Liao G, Yang J, Tang L, Li C. Am J Med Sci. 2013 Oct;346(4):303-7. Doi: 10.1097/Maj.0B013E318272087A. Focused Ultrasound Therapy For Vulvar Intraepithelial Neoplasia In A Mice Model.
Ling S, Deng D, Mo Y, Zhang X, Guan X, Wei Q. Cell Biochem Biophys. 2014 Sep;70(1):629-33. Doi: 10.1007/S12013-014-9966-8. Correlations Between Ct Perfusion Parameters And Vascular Endothelial Growth Factor Expression And Microvessel Density In Im...
Liu Y, Liu Y, Jiang H, Xu L, Cheng Y, Wang Pg, Wang F. Carbohydr Polym. 2014 Jun 15;106:42-8. Doi: 10.1016/J.Carbpol.2014.01.088. Epub 2014 Feb 4. Preparation, Antiangiogenic And Antitumoral Activities Of The Chemically Sulfated Glucan From Phelli...
Hou C, Kong J, Liang Y, Huang H, Wen H, Zheng X, Wu L, Chen Y. Cell Mol Immunol. 2015 Jul;12(4):409-23. Doi: 10.1038/Cmi.2014.60. Epub 2014 Aug 25. Hmgb1 Contributes To Allergen-Induced Airway Remodeling In A Murine Model Of Chronic Asthma By Modu...
Wang Z, Lv J, Zhang T. Oncol Rep. 2015 May;33(5):2468-76. Doi: 10.3892/Or.2015.3853. Epub 2015 Mar 16. Combination Of Il-24 And Cisplatin Inhibits Angiogenesis And Lymphangiogenesis Of Cervical Cancer Xenografts In A Nude Mouse Model By Inhibiting...
Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.
Ye Y, Huang C, Jiang L, Shen X, Zhu S, Rao Y, Wang J, Zhang Q. Biol Pharm Bull. 2012;35(3):355-61. Huanglian-Jie-Du-Tang Extract Protects Against Chronic Brain Injury After Focal Cerebral Ischemia Via Hypoxia-Inducible-Factor-1??-Regulated Vascula...
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.
Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.
Zhang Lh, Jia Yl, Lin Xx, Zhang Hq, Dong Xw, Zhao Jm, Shen J, Shen Hj, Li Ff, Yan Xf, Li W, Zhao Yq, Xie Qm. Biochim Biophys Acta. 2013 Aug;1830(8):4148-59. Doi: 10.1016/J.Bbagen.2013.04.008. Epub 2013 Apr 11. Ad-1, A Novel Ginsenoside Derivative,...
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...
Zhou J, Wang L, Xu X, Tu Y, Qin S, Yin Y. Oncol Lett. 2012 Nov;4(5):976-980. Epub 2012 Aug 8. Antitumor Activity Of Endostar Combined With Radiation Against Human Nasopharyngeal Carcinoma In Mouse Xenograft Models.
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...
Duan J, Zhang Y, Han S, Chen Y, Li B, Liao M, Chen W, Deng X, Zhao J, Huang B. Int J Pharm. 2010 Nov 15;400(1-2):211-20. Doi: 10.1016/J.Ijpharm.2010.08.033. Epub 2010 Sep 8. Synthesis And In Vitro/In Vivo Anti-Cancer Evaluation Of Curcumin-Loaded ...
Xie Y, Chen S, Wang Ch, Tang Cw. Cancer Biol Ther. 2011 Jul 1;12(1):86-92. Doi: 10.4161/Cbt.12.1.15730. Epub 2011 Jul 1. Som230 Combined With Celecoxib Prolongs The Survival In Nude Mice With Hepg-2 Xenografts.
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Zhao X, Sun X, Li Xl. Asian Pac J Cancer Prev. 2012;13(6):2873-7. Expression And Clinical Significance Of Stat3, P-Stat3, And Vegf-C In Small Cell Lung Cancer.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vegfa antibody, vegf-a antibody