Anti-VEGF/VEGFA Antibody

Boster Bio Anti-VEGF/VEGFA Antibody catalog # PA1080. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 107 publication(s).

Product Info Summary

SKU: PA1080
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-VEGF/VEGFA Antibody

See all VEGFA products

SKU/Catalog Number







Boster Bio Anti-VEGF/VEGFA Antibody catalog # PA1080. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF/VEGFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1080)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF (33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1080 is reactive to VEGFA in Human, Mouse, Rat


PA1080 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For VEGFA (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Vascular endothelial growth factor A




PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VEGFA, check out the VEGFA Infographic

VEGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VEGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1080 has been cited in 107 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sun,S.,Du,Y.,Li,S.,Gao,B.,Xia,R.,Cao,W. ... Zhu,E.(2021).Anti‑inflammatory activity of different isolated sites of Chloranthus serratus in complete Freund‘s adjuvant‑induced arthritic rats.Experimental and Therapeutic Medicine,22,848.
Species: Rat
PA1080 usage in article: APP:IHC, SAMPLE:JOINT TISSUE, DILUTION:1:100

Qu X,Fang Y,Zhuang S,Zhang Y.Micro-RNA miR-542-3p suppresses decidualization by targeting ILK pathways in human endometrial stromal cells.Sci Rep.2021 Mar 30;11(1):7186.doi:10.1038/s41598-021-85295-2.PMID:33785768;PMCID:PMC8009905.
Species: Human,Mouse
PA1080 usage in article: APP:WB, SAMPLE:HESCS, DILUTION:1:200

Sun S,Li S,Du Y,Wu C,Zhang M,Li J,Zhang X.Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats.Pharm Biol.2020 Dec;58(1):528-537.doi:10.1080/13880209.2020.1767159.PMID:32503379.
Species: Rat
PA1080 usage in article: APP:IHC, SAMPLE:ANKLE JOINT, DILUTION:1:100

Huilin Guo,Huimin Li,Yaping Feng,Jin Ke,Wei Fang,Cheng Li,Xing Long,Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study,Oral Surgery,Oral Medicine,Oral Pathology and Oral Radiology,2020,ISSN 2212-4403,
Species: Human

Huilin Guo,Huimin Li,Yaping Feng,Jin Ke,Wei Fang,Cheng Li,Xing Long,Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study,Oral Surgery,Oral Medicine,Oral Pathology and Oral Radiology,2020,ISSN 2212-4403,
Species: Human

Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human

Hu H,Zhu S,Tong Y,Huang G,Tan B,Yang L.Antitumor activity of triptolide in SKOV3 cells and SKOV3/DDP in vivo and in vitro.Anticancer Drugs.2020 Jun;31(5):483-491.doi:10.1097/CAD.0000000000000894. PMID:31972592.
Species: Human,Mouse

Erpolat OP,Demircan NV,Sarıbas GS,Kuzucu P,Senturk E,Elmas C,Borcek A,Kurt G.A Comparison of Ramipril and Bevacizumab to Mitigate Radiation-Induced Brain Necrosis: An Experimental Study.World Neurosurg. 2020 Dec;144:e210-e220.doi:10.1016/j.wneu.2020.08.08
Species: Rat

Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration

Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis

Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma

Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model

Proper autophagy is indispensable for angiogenesis during chick embryo development

VEGFR-2 conformational switch in response to ligand binding

Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells

Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway

Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress

Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction

Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice

Insufficient radiofrequency ablation promotes human hepatoma SMMC7721 cell proliferation by stimulating vascular endothelial growth factor overexpression

Ectopic expression of AP-2? transcription factor suppresses glioma progression

microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma

Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer

HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head

Roxarsone induces angiogenesis via PI3K/Akt signaling

Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1

Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization

A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair

Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Correlation and Significance of Urinary Soluble Fas and Vascular Endothelial Growth Factor in Bladder Urothelial Cancer

Factors influencing improvement of visual field after trans-sphenoidal resection of pituitary macroadenomas: a retrospective cohort study

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells

Ning D, Zhang WK, Tian H, Li XJ, Liu M, Li YS, Tang HB. J Ophthalmol. 2017;2017:9760501. doi: 10.1155/2017/9760501. Epub 2017 Oct 12. The Involvement of β-Catenin/COX-2/VEGF Axis in NMDA-Caused Retinopathy

Ma X, Li Z, Li T, Zhu L, Li Z, Tian N. Am J Transl Res. 2017 Nov 15;9(11):5012-5021. eCollection 2017. Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glio...

Yan-Ping D, Xiao-Qin G, Xiao Ping M, Ying Quan Y. Biomed Res Int. 2017;2017:2597256. doi: 10.1155/2017/2597256. Epub 2017 Jul 16. Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

Qin JH, Ke ZY, Zhou Q, Wang L, Liang Y, Wang YM, Yang T, Gao X, Ye J, Kumar R, Wang RA,. Med Sci Monit. 2017 Aug 15;23:3932-3941. Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice

Li P, Chen H, Chen S, Mo X, Li T, Xiao B, Yu R, Guo J. Br J Cancer. 2017 Feb 28;116(5):626-633. doi: 10.1038/bjc.2016.451. Epub 2017 Jan 12 Circular RNA 0000096 affects cell growth and migration in gastric cancer

Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010

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Zhang B, Zhang Y, Yao G, Gao J, Yang B, Zhao Y, Rao Z, Gao J. Clinics (Sao Paulo). 2012 Aug;67(8):901-6. M2-Polarized Macrophages Promote Metastatic Behavior Of Lewis Lung Carcinoma Cells By Inducing Vascular Endothelial Growth Factor-C Expression.

Xuebing P, Tinchiu L, Enlan X, Jing L, Xiaowu H. Eur J Obstet Gynecol Reprod Biol. 2011 Nov;159(1):198-203. Doi: 10.1016/J.Ejogrb.2011.06.036. Epub 2011 Jul 2. Is Endometrial Polyp Formation Associated With Increased Expression Of Vascular Endothe...

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Li X, Wang H, Lu X, Di B. Exp Ther Med. 2010 Nov;1(6):947-953. Epub 2010 Sep 29. Silencing Stat3 With Short Hairpin Rna Enhances Radiosensitivity Of Human Laryngeal Squamous Cell Carcinoma Xenografts In Vivo.

Zhang S, Yu H, Zhang L. Oncol Lett. 2010 May;1(3):453-456. Epub 2010 May 1. Role Of Vascular Endothelial Growth Factor Receptor-3/Flt-4 In Early-Stage Cervical Cancer.

Tang C, Wu X, Ye L, Xie X, Wang G. Biocell. 2012 Dec;36(3):97-103. Effects Of Rotational Culture On Morphology, Nitric Oxide Production And Cell Cycle Of Endothelial Cells.

Zhao W, Shen H, Yuan F, Li G, Sun Y, Shi Z, Zhang Y, Wang Z. Eur J Histochem. 2009 Jan-Mar;53(1):53-60. Induction Stage-Dependent Expression Of Vascular Endothelial Growth Factor And Aquaporin-1 In Diethylstilbestrol-Treated Rat Pituitary.

Liang Ql, Wang Br, Chen Gq, Li Gh, Xu Yy. Med Oncol. 2010 Dec;27(4):1164-70. Doi: 10.1007/S12032-009-9354-1. Epub 2009 Nov 12. Clinical Significance Of Vascular Endothelial Growth Factor And Connexin43 For Predicting Pancreatic Cancer Clinicopatho...

Zhan Cy, Yuan Tm, Sun Y, Yu Hm. J Matern Fetal Neonatal Med. 2011 Feb;24(2):213-22. Doi: 10.3109/14767051003758895. Epub 2010 May 17. Early Gestational Intrauterine Infection Induces Postnatal Lung Inflammation And Arrests Lung Development In A Ra...

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Jiang X, Ge H, Zhou C, Chai X, Ren Qs. Lasers Med Sci. 2012 May;27(3):599-606. Doi: 10.1007/S10103-011-0996-9. Epub 2011 Oct 1. The Role Of Vascular Endothelial Growth Factor In Fractional Laser Resurfacing With The Carbon Dioxide Laser.

Guo Q, Jin J, Yuan Jx, Zeifman A, Chen J, Shen B, Huang J. Biochem Biophys Res Commun. 2011 Oct 7;413(4):630-6. Doi: 10.1016/J.Bbrc.2011.09.015. Epub 2011 Sep 12. Vegf, Bcl-2 And Bad Regulated By Angiopoietin-1 In Oleic Acid Induced Acute Lung Inj...

Zhang T, Liu M, Wang C, Lin C, Sun Y, Jin D. Anticancer Res. 2011 Nov;31(11):3859-63. Down-Regulation Of Mir-206 Promotes Proliferation And Invasion Of Laryngeal Cancer By Regulating Vegf Expression.

Zhang W, Yan H. Exp Eye Res. 2012 Dec;105:1-8. Doi: 10.1016/J.Exer.2012.09.014. Epub 2012 Oct 8. Simvastatin Increases Circulating Endothelial Progenitor Cells And Reduces The Formation And Progression Of Diabetic Retinopathy In Rats.

Jia Y, Wu J, Chen L, Liao G, Yang J, Tang L, Li C. Am J Med Sci. 2013 Oct;346(4):303-7. Doi: 10.1097/Maj.0B013E318272087A. Focused Ultrasound Therapy For Vulvar Intraepithelial Neoplasia In A Mice Model.

Ling S, Deng D, Mo Y, Zhang X, Guan X, Wei Q. Cell Biochem Biophys. 2014 Sep;70(1):629-33. Doi: 10.1007/S12013-014-9966-8. Correlations Between Ct Perfusion Parameters And Vascular Endothelial Growth Factor Expression And Microvessel Density In Im...

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Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.

Ye Y, Huang C, Jiang L, Shen X, Zhu S, Rao Y, Wang J, Zhang Q. Biol Pharm Bull. 2012;35(3):355-61. Huanglian-Jie-Du-Tang Extract Protects Against Chronic Brain Injury After Focal Cerebral Ischemia Via Hypoxia-Inducible-Factor-1??-Regulated Vascula...

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Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.

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Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.

Lu Ql, Liu J, Zhu Xl, Xu Wj. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):359-62. Doi: 10.1007/S11596-014-1283-3. Epub 2014 Jun 18. Expression Of Nerve Growth Factor And Hypoxia Inducible Factor-1?? And Its Correlation With Angiogenesis I...

Yu Zy, Bai Yn, Luo Lx, Wu H, Zeng Y. Mol Med Rep. 2013 Jul;8(1):287-93. Doi: 10.3892/Mmr.2013.1493. Epub 2013 May 28. Expression Of Microrna-150 Targeting Vascular Endothelial Growth Factor-A Is Downregulated Under Hypoxia During Liver Regeneration.

Zhang L, Wu X, Luo C, Chen X, Yang L, Tao J, Shi J. Mol Med Rep. 2013 Jul;8(1):272-6. Doi: 10.3892/Mmr.2013.1458. Epub 2013 May 8. The 786-0 Renal Cancer Cell-Derived Exosomes Promote Angiogenesis By Downregulating The Expression Of Hepatocyte Cel...

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Zhao M, Shi X, Liang J, Miao Y, Xie W, Zhang Y, Li X. Exp Eye Res. 2011 Dec;93(6):921-6. Doi: 10.1016/J.Exer.2011.10.013. Epub 2011 Nov 4. Expression Of Pro- And Anti-Angiogenic Isoforms Of Vegf In The Mouse Model Of Oxygen-Induced Retinopathy.

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Yang W, Zhang Y, Yu J, Li S. Eur J Surg Oncol. 2010 May;36(5):501-6. Doi: 10.1016/J.Ejso.2010.01.007. Epub 2010 Feb 23. The Low Expression Of Cd80 Correlated With The Vascular Endothelial Growth Factor In Esophageal Cancer Tissue.

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Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...

Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.

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18 Customer Q&As for Anti-VEGF/VEGFA Antibody


What is the peptide coverage for PA1080?

Verified customer

Asked: 2020-10-09


The immunogen sequence of the Anti-VEGF/VEGFA Antibody (PA1080) is 33-48aa VDIFQEYPDEIEYIFK.

Boster Scientific Support

Answered: 2020-10-09


Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-VEGF/VEGFA antibody PA1080. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-31


Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-31


We bought anti-VEGF/VEGFA antibody for IHC on mammary gland in a previous experiment. I am using mouse, and We want to use the antibody for WB next. We are interested in examining mammary gland as well as hemangioendothelioma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-02-17


I took a look at the website and datasheets of our anti-VEGF/VEGFA antibody and it appears that PA1080 has been validated on mouse in both IHC and WB. Thus PA1080 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website to find out more information about them.

Boster Scientific Support

Answered: 2020-02-17


I am interested in to test anti-VEGF/VEGFA antibody PA1080 on human lung for research purposes, then I may be interested in using anti-VEGF/VEGFA antibody PA1080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-07


The products we sell, including anti-VEGF/VEGFA antibody PA1080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-07


I was wanting to use your anti-VEGF/VEGFA antibody for IHC for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?

Verified Customer

Verified customer

Asked: 2020-01-30


You can see on the product datasheet, PA1080 anti-VEGF/VEGFA antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-30


I am interested in using your anti-VEGF/VEGFA antibody for eye photoreceptor cell development studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-28


I appreciate your inquiry. This PA1080 anti-VEGF/VEGFA antibody is tested on lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-28


Will anti-VEGF/VEGFA antibody PA1080 work on bovine WB with kidney?

Verified Customer

Verified customer

Asked: 2020-01-01


Our lab technicians have not validated anti-VEGF/VEGFA antibody PA1080 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-VEGF/VEGFA antibody PA1080 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-01


Here is the WB image, lot number and protocol we used for lung using anti-VEGF/VEGFA antibody PA1080. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-26


Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-26


We are currently using anti-VEGF/VEGFA antibody PA1080 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-21


The anti-VEGF/VEGFA antibody (PA1080) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-21


Is a blocking peptide available for product anti-VEGF/VEGFA antibody (PA1080)?

Verified Customer

Verified customer

Asked: 2019-10-17


We do provide the blocking peptide for product anti-VEGF/VEGFA antibody (PA1080). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-17


We have observed staining in mouse kidney. Any tips? Is anti-VEGF/VEGFA antibody supposed to stain kidney positively?

Verified Customer

Verified customer

Asked: 2019-07-23


According to literature kidney does express VEGFA. According to, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2019-07-23


I see that the anti-VEGF/VEGFA antibody PA1080 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-05


You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-05


Is there a BSA free version of anti-VEGF/VEGFA antibody PA1080 available?

Verified Customer

Verified customer

Asked: 2017-10-17


Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/VEGFA antibody PA1080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-17


Our team were well pleased with the WB result of your anti-VEGF/VEGFA antibody. However we have seen positive staining in lung secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-14


According to literature, lung does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2017-08-14


Can you help my question with product PA1080, anti-VEGF/VEGFA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Baker

Verified customer

Asked: 2017-05-25


We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1080 anti-VEGF/VEGFA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-25


Is this PA1080 anti-VEGF/VEGFA antibody reactive to the isotypes of VEGFA?

P. Kulkarni

Verified customer

Asked: 2016-01-26


The immunogen of PA1080 anti-VEGF/VEGFA antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-01-26


Would PA1080 anti-VEGF/VEGFA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Krishna

Verified customer

Asked: 2014-08-07


You can see on the product datasheet, PA1080 anti-VEGF/VEGFA antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-08-07


Does anti-VEGF/VEGFA antibody PA1080 work for IHC with lung?

K. Roberts

Verified customer

Asked: 2013-03-18


According to the expression profile of lung, VEGFA is highly expressed in lung. So, it is likely that anti-VEGF/VEGFA antibody PA1080 will work for IHC with lung.

Boster Scientific Support

Answered: 2013-03-18


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