Anti-VEGF/VEGFA Antibody

VEGFA antibody

Boster Bio Anti-VEGF/VEGFA Antibody catalog # PA1080. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1080
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-VEGF/VEGFA Antibody

View all VEGFA Antibodies

SKU/Catalog Number

PA1080

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF/VEGFA Antibody catalog # PA1080. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF/VEGFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1080)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF (33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1080 is reactive to VEGFA in Human, Mouse, Rat

Applications

PA1080 is guaranteed for IHC, WB Boster Guarantee

Background of VEGFA

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)

Uniprot ID

P15692

Gene ID

7422

Gene Name

VEGFA

Full Name

Vascular endothelial growth factor A

Weight

27.042kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VEGFA, check out the VEGFA Infographic

VEGFA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VEGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1080 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fentanyl inhibits the progression of human gastric carcinoma MGC-803 cells by modulating NF-κB-dependent gene expression in vivo

Sun,S.,Du,Y.,Li,S.,Gao,B.,Xia,R.,Cao,W. ... Zhu,E.(2021).Anti‑inflammatory activity of different isolated sites of Chloranthus serratus in complete Freund‘s adjuvant‑induced arthritic rats.Experimental and Therapeutic Medicine,22,848.https://doi.org/10.3892/etm.2021.10280
Species: Rat
PA1080 usage in article: APP:IHC, SAMPLE:JOINT TISSUE, DILUTION:1:100

Qu X,Fang Y,Zhuang S,Zhang Y.Micro-RNA miR-542-3p suppresses decidualization by targeting ILK pathways in human endometrial stromal cells.Sci Rep.2021 Mar 30;11(1):7186.doi:10.1038/s41598-021-85295-2.PMID:33785768;PMCID:PMC8009905.
Species: Human,Mouse
PA1080 usage in article: APP:WB, SAMPLE:HESCS, DILUTION:1:200

Sun S,Li S,Du Y,Wu C,Zhang M,Li J,Zhang X.Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats.Pharm Biol.2020 Dec;58(1):528-537.doi:10.1080/13880209.2020.1767159.PMID:32503379.
Species: Rat
PA1080 usage in article: APP:IHC, SAMPLE:ANKLE JOINT, DILUTION:1:100

Huilin Guo,Huimin Li,Yaping Feng,Jin Ke,Wei Fang,Cheng Li,Xing Long,Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study,Oral Surgery,Oral Medicine,Oral Pathology and Oral Radiology,2020,ISSN 2212-4403,
Species: Human
PA1080 usage in article: APP:IHC, SAMPLE:CONDYLAR TISSUES, DILUTION:1:50

Huilin Guo,Huimin Li,Yaping Feng,Jin Ke,Wei Fang,Cheng Li,Xing Long,Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study,Oral Surgery,Oral Medicine,Oral Pathology and Oral Radiology,2020,ISSN 2212-4403,
Species: Human
PA1080 usage in article: APP:IHC, SAMPLE:CONDYLAR TISSUES, DILUTION:1:50

Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human
PA1080 usage in article: APP:IHC, SAMPLE:PCA TISSUE, DILUTION:NA

Hu H,Zhu S,Tong Y,Huang G,Tan B,Yang L.Antitumor activity of triptolide in SKOV3 cells and SKOV3/DDP in vivo and in vitro.Anticancer Drugs.2020 Jun;31(5):483-491.doi:10.1097/CAD.0000000000000894. PMID:31972592.
Species: Human,Mouse
PA1080 usage in article: APP:IHC, SAMPLE:OVARIAN TUMOR TISSUE, DILUTION:1:100

Erpolat OP,Demircan NV,Sarıbas GS,Kuzucu P,Senturk E,Elmas C,Borcek A,Kurt G.A Comparison of Ramipril and Bevacizumab to Mitigate Radiation-Induced Brain Necrosis: An Experimental Study.World Neurosurg. 2020 Dec;144:e210-e220.doi:10.1016/j.wneu.2020.08.08
Species: Rat

Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration

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18 Customer Q&As for Anti-VEGF/VEGFA Antibody

Question

What is the peptide coverage for PA1080?

Verified customer

Asked: 2020-10-09

Answer

The immunogen sequence of the Anti-VEGF/VEGFA Antibody (PA1080) is 33-48aa VDIFQEYPDEIEYIFK.

Boster Scientific Support

Answered: 2020-10-09

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-VEGF/VEGFA antibody PA1080. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-31

Question

We bought anti-VEGF/VEGFA antibody for IHC on mammary gland in a previous experiment. I am using mouse, and We want to use the antibody for WB next. We are interested in examining mammary gland as well as hemangioendothelioma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

I took a look at the website and datasheets of our anti-VEGF/VEGFA antibody and it appears that PA1080 has been validated on mouse in both IHC and WB. Thus PA1080 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-17

Question

I am interested in to test anti-VEGF/VEGFA antibody PA1080 on human lung for research purposes, then I may be interested in using anti-VEGF/VEGFA antibody PA1080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

The products we sell, including anti-VEGF/VEGFA antibody PA1080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-07

Question

I was wanting to use your anti-VEGF/VEGFA antibody for IHC for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

You can see on the product datasheet, PA1080 anti-VEGF/VEGFA antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-30

Question

I am interested in using your anti-VEGF/VEGFA antibody for eye photoreceptor cell development studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

I appreciate your inquiry. This PA1080 anti-VEGF/VEGFA antibody is tested on lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-28

Question

Will anti-VEGF/VEGFA antibody PA1080 work on bovine WB with kidney?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

Our lab technicians have not validated anti-VEGF/VEGFA antibody PA1080 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-VEGF/VEGFA antibody PA1080 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-01

Question

Here is the WB image, lot number and protocol we used for lung using anti-VEGF/VEGFA antibody PA1080. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-26

Question

We are currently using anti-VEGF/VEGFA antibody PA1080 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

The anti-VEGF/VEGFA antibody (PA1080) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-21

Question

Is a blocking peptide available for product anti-VEGF/VEGFA antibody (PA1080)?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

We do provide the blocking peptide for product anti-VEGF/VEGFA antibody (PA1080). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-17

Question

We have observed staining in mouse kidney. Any tips? Is anti-VEGF/VEGFA antibody supposed to stain kidney positively?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

According to literature kidney does express VEGFA. According to Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2019-07-23

Question

I see that the anti-VEGF/VEGFA antibody PA1080 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-05

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-05

Question

Is there a BSA free version of anti-VEGF/VEGFA antibody PA1080 available?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/VEGFA antibody PA1080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-17

Question

Our team were well pleased with the WB result of your anti-VEGF/VEGFA antibody. However we have seen positive staining in lung secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

According to literature, lung does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2017-08-14

Question

Can you help my question with product PA1080, anti-VEGF/VEGFA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Baker

Verified customer

Asked: 2017-05-25

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1080 anti-VEGF/VEGFA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-25

Question

Is this PA1080 anti-VEGF/VEGFA antibody reactive to the isotypes of VEGFA?

P. Kulkarni

Verified customer

Asked: 2016-01-26

Answer

The immunogen of PA1080 anti-VEGF/VEGFA antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-01-26

Question

Would PA1080 anti-VEGF/VEGFA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Krishna

Verified customer

Asked: 2014-08-07

Answer

You can see on the product datasheet, PA1080 anti-VEGF/VEGFA antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-08-07

Question

Does anti-VEGF/VEGFA antibody PA1080 work for IHC with lung?

K. Roberts

Verified customer

Asked: 2013-03-18

Answer

According to the expression profile of lung, VEGFA is highly expressed in lung. So, it is likely that anti-VEGF/VEGFA antibody PA1080 will work for IHC with lung.

Boster Scientific Support

Answered: 2013-03-18

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Conjugation

Total: $315

SKU:PA1080

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PA1080
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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