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Product Info Summary
| SKU: | M00045 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Dog, Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody
SKU/Catalog Number
M00045
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody (Catalog # M00045). Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Dog.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00045)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM225
Isotype
IgG1, kappa
Immunogen
Human VEGF189 recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00045 is reactive to VEGFA in Dog, Human, Mouse, Rabbit, Rat
Applications
M00045 is guaranteed for IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
27.042kDa
Background of VEGFA
This monoclonal antibody recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Anti-VEGF Mouse Monoclonal Antibody (SPM225).
Protein Target Info & Infographic
Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)
Gene Name
VEGFA
Full Name
Vascular endothelial growth factor A
Weight
27.042kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VEGFA, check out the VEGFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VEGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody (M00045)
Hello CJ!
M00045 has been cited in 44 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A
Serum vascular endothelial growth factor and angiogenesis are related to the prognosis of patients with gastrointestinal stromal tumors
Inhibitory effect of extract of fungi of Huaier on hepatocellular carcinoma cells
Liang M,Chen Z,Wang F,Liu L,Wei R,Zhang M.Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.J Biomed Mater Res B Appl Biomater.2019 Jul;107(5):1471-1482.doi:10.1002/jbm.b.34239.Epub 2018 Oct 8.PMID:30296361.
Species: Rat
M00045 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:NA
Junyi Tang,Chunyan Liu,Bing Xu,Dawei Wang,Zhenshen Ma,Xiaotian Chang,ARHGEF10L contributes to liver tumorigenesis through RhoA-ROCK1 signaling and the epithelial-mesenchymal transition,Experimental Cell Research,Volume 374,Issue 1,2019,Pages 46-68,ISSN 0014-4827,https://doi.org/10.1016/j.yexcr.2018.11.007.
Species: Human,Mouse
M00045 usage in article: APP:WB, SAMPLE:HEPATIC TISSUE, DILUTION:NA
Shao JB,Li Z,Zhang N,Yang F,Gao W,Sun ZG.Hypoxia-inducible factor 1α in combination with vascular endothelial growth factor could predict the prognosis of postoperative patients with oesophageal squamous cell cancer.Pol J Pathol.2019;70(2):84-90.doi:10.5114/pjp.2019. 87100.PMID:31556558.
Species: Human
M00045 usage in article: APP:IHC, SAMPLE:OSCC TISSUE, DILUTION:1:100
Sun S,Li S,Du Y,Wu C,Zhang M,Li J,Zhang X.Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats.Pharm Biol.2020 Dec;58(1):528-537.doi:10.1080/13880209.2020.1767159.PMID:32503379.
Species: Rat
M00045 usage in article: APP:IHC, SAMPLE:ANKLE JOINT, DILUTION:1:100
Huilin Guo,Huimin Li,Yaping Feng,Jin Ke,Wei Fang,Cheng Li,Xing Long,Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study,Oral Surgery,Oral Medicine,Oral Pathology and Oral Radiology,2020,ISSN 2212-4403,
Species: Human
M00045 usage in article: APP:IHC, SAMPLE:CONDYLAR TISSUES, DILUTION:1:50
Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human
M00045 usage in article: APP:IHC, SAMPLE:PCA TISSUE, DILUTION:NA
Erpolat OP,Demircan NV,Sarıbas GS,Kuzucu P,Senturk E,Elmas C,Borcek A,Kurt G.A Comparison of Ramipril and Bevacizumab to Mitigate Radiation-Induced Brain Necrosis: An Experimental Study.World Neurosurg. 2020 Dec;144:e210-e220.doi:10.1016/j.wneu.2020.08.08
Species: Rat
M00045 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody
Question
We are currently using anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody M00045 for canine tissue, and we are content with the IF results. The species of reactivity given in the datasheet says canine, human, mouse, rabbit, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody (M00045) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
Our lab were well pleased with the WB result of your anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody. However we have seen positive staining in mammary gland secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?
F. Mitchell
Verified customer
Asked: 2018-08-21
Answer
According to literature, mammary gland does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-08-21
Question
We have seen staining in rat retina. Any tips? Is anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody supposed to stain retina positively?
V. Rodriguez
Verified customer
Asked: 2016-03-23
Answer
From literature retina does express VEGFA. From Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2016-03-23
Question
We bought anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody for IHC-P on hemangioendothelioma in the past. I am using rabbit, and We want to use the antibody for IF next. I am interested in examining hemangioendothelioma as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for IF?
E. Johnson
Verified customer
Asked: 2015-11-24
Answer
I have checked the website and datasheets of our anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal antibody and it appears that M00045 has been tested on rabbit in both IHC-P and IF. Thus M00045 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rabbit even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-11-24
