Click image to see more details
Product Info Summary
| SKU: | M00235-5 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
| Host: | Chicken |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Vimentin Antibody
SKU/Catalog Number
M00235-5
Size
100ul
Description
Boster Bio Anti-Vimentin Antibody catalog # M00235-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Stable at 4 ° C for at least one year
Cite This Product
Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-5)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00235-5 is reactive to VIM in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Applications
M00235-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53.652kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10000.
IF/ICC and IHC: 1:5000.
Validation Images & Assay Conditions
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Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, M00235-5, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. M00235-5 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
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Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin, M00235-5, dilution 1:10,000 in green, and costained with Boster mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Protein Target Info & Infographic
Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)
Gene Name
VIM
Full Name
Vimentin
Weight
53.652kDa
Superfamily
intermediate filament family
Alternative Names
FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIM, check out the VIM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vimentin Antibody (M00235-5)
Hello CJ!
M00235-5 has been cited in 32 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues
Liu R,Xu B,Yu S,Zhang J,Sun H,Liu C,Lu F,Pan Q,Zhang X.Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae.Front Microbiol.2020 Apr 3;11:576.doi:10.3389/fmicb.2020.00576.PMID:323
Species: Chicken
M00235-5 usage in article: APP:IF, SAMPLE:CSF, DILUTION:NA
Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
Species: Human,Mouse
M00235-5 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
M00235-5 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
Species: Human
M00235-5 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties
Expression of E-cadherin and vimentin in oral squamous cell carcinoma
%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Vimentin Antibody
Question
We need using your anti-Vimentin antibody for striated muscle contraction studies. Has this antibody been tested with western blotting on neuronal lineage cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
I appreciate your inquiry. This M00235-5 anti-Vimentin antibody is tested on neuronal lineage cells. It is guaranteed to work for IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Vimentin antibody M00235-5. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-Vimentin antibody M00235-5 for chicken tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-Vimentin antibody (M00235-5) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
I was wanting to use your anti-Vimentin antibody for WB for pig liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for pig liver identification?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
As indicated on the product datasheet, M00235-5 anti-Vimentin antibody has been tested for IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-07
Question
We are interested in to test anti-Vimentin antibody M00235-5 on pig liver for research purposes, then I may be interested in using anti-Vimentin antibody M00235-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The products we sell, including anti-Vimentin antibody M00235-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-11
Question
Would M00235-5 anti-Vimentin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
It shows on the product datasheet, M00235-5 anti-Vimentin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-07
Question
My colleagues were satisfied with the WB result of your anti-Vimentin antibody. However we have seen positive staining in cajal-retzius cell fetal brain cortex cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
From literature, cajal-retzius cell fetal brain cortex does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2019-06-11
Question
Is this M00235-5 anti-Vimentin antibody reactive to the isotypes of VIM?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The immunogen of M00235-5 anti-Vimentin antibody is Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-31
Question
Please see the WB image, lot number and protocol we used for liver using anti-Vimentin antibody M00235-5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-03
Question
I see that the anti-Vimentin antibody M00235-5 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-28
Question
Do you have a BSA free version of anti-Vimentin antibody M00235-5 available?
J. Edwards
Verified customer
Asked: 2018-01-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vimentin antibody M00235-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-04
Question
Is a blocking peptide available for product anti-Vimentin antibody (M00235-5)?
H. Anderson
Verified customer
Asked: 2017-12-14
Answer
We do provide the blocking peptide for product anti-Vimentin antibody (M00235-5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-14
Question
Would anti-Vimentin antibody M00235-5 work for WB with liver?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
According to the expression profile of liver, VIM is highly expressed in liver. So, it is likely that anti-Vimentin antibody M00235-5 will work for WB with liver.
Boster Scientific Support
Answered: 2017-10-31
Question
Our lab used your anti-Vimentin antibody for IHC on lymphoblast last year. I am using human, and I plan to use the antibody for WB next. We need examining lymphoblast as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
T. Johnson
Verified customer
Asked: 2017-01-30
Answer
I looked at the website and datasheets of our anti-Vimentin antibody and it seems that M00235-5 has been tested on human in both IHC and WB. Thus M00235-5 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-01-30
Question
My question regarding product M00235-5, anti-Vimentin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Carter
Verified customer
Asked: 2014-05-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00235-5 anti-Vimentin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-09
Question
We have seen staining in mouse cajal-retzius cell fetal brain cortex. Do you have any suggestions? Is anti-Vimentin antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
D. Roberts
Verified customer
Asked: 2014-04-08
Answer
From what I have seen in literature cajal-retzius cell fetal brain cortex does express VIM. From what I have seen in Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2014-04-08
