SKU MA1102
Size 100μg/vial
Reactivity Human, Pig, Rabbit, Rat
Clonality Monoclonal
Clone Number V9
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Vimentin Antibody (Monoclonal, V9)
SKU/Catalog Number MA1102
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Vimentin, vimentin (VIM) detection. Tested with WB, IHC-P in Human;rat;rabbit;pig. No cross reactivity with other proteins.
Cite This Product Anti-Vimentin Antibody (Monoclonal, V9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1102)
Host Mouse
Immunogen Pig eye lens vimentin.
Reactivity Human, Pig, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1.5-2.5μg/ml, Human, rabbit, pig, rat, By Heat
Western blot, 0.5-1μg/ml, Human, rabbit, pig, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1102 1 IHC anti vimentin antibody monoclonal v9.jpg

Figure 1. IHC analysis of Vimentin using anti- Vimentin antibody (MA1102).
Vimentin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Vimentin Antibody (MA1102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/m/a/ma1102 2.jpg

Figure 2. Western blot analysis of Vimentin using anti-Vimentin antibody (MA1102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # MA1102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54KD. The expected band size for Vimentin is at 54KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P31000
Gene Name Vim
Protein Name Vimentin
Tissue Specificity VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Alternative Names Vimentin;Vim;
Subcellular Localization Nucleus matrix . Cytoplasm .
Molecular Weight 53733 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .
Background The VIM gene was one of many that Gieser and Swaroop(1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

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Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

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Troubleshooting

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Publications

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Silencing SOX2 Induced Mesenchymal-Epithelial Transition and Its Expression Predicts Liver and Lymph Node Metastasis of CRC Patients
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Heat Shock Protein 72 Enhances Autophagy as a Protective Mechanism in Lipopolysaccharide-Induced Peritonitis in Rats
miR-200a Regulates Epithelial-Mesenchymal to Stem-like Transition via ZEB2 and ?-Catenin Signaling
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vimentin antibody, vim antibody