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Product Info Summary
| SKU: | A32320 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-VISTA VSIR Antibody
View all VISTA/B7-H5/PD-1H Antibodies
SKU/Catalog Number
A32320
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VISTA VSIR Antibody (Catalog # A32320). Tested in ELISA, WB, IHC, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VISTA VSIR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32320)
Host
Rabbit
Contents
VISTA Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA. The immunogen is located within the last 60 amino acids of VISTA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
It is predicted to not cross-react with other members of the cavin family.
Reactive Species
A32320 is reactive to VSIR in Human
Applications
A32320 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.908kDa
Background of VISTA/B7-H5/PD-1H
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VISTA antibody can be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. For Immunocytochemistry start at 1 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunohistochemistry of VISTA in human tonsil tissue with B7-H5 antibody at 2 μg/ml.
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Immunofluorescence of VISTA in human tonsil tissue with B7-H5 antibody at 20 μg/ml.
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Immunocytochemistry of VISTA in overexpressing HEK293 cells with VISTA antibody at 1 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For VSIR (Source: Uniprot.org, NCBI)
Gene Name
VSIR
Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
Weight
33.908kDa
Alternative Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR VSIR B7-H5, B7H5, C10orf54, DD1alpha, Dies1, GI24, PD-1H, PP2135, SISP1, VISTA V-set immunoregulatory receptor V-type immunoglobulin domain-containing suppressor of T-cell activation|Death Domain1alpha|PDCD1 homolog|V-domain Ig suppressor of T cell activation|V-set domain-containing immunoregulatory receptor|platelet receptor GI24|sisp-1|stress-induced secreted protein-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VSIR, check out the VSIR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VSIR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VISTA VSIR Antibody (A32320)
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No publications found for A32320
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Customer Reviews
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