|Applications||Flow Cytometry, IF, IHC, WB|
|Product Name||Anti-WASL Monoclonal Antibody|
|Storage & Handling||Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.|
|Description||Rabbit IgG Monoclonal antibody for O00401 detection. Tested positive for WB IHC IF/IF FC in Human Mouse|
|Cite This Product||Anti-WASL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05438)|
|Immunogen||A synthesized peptide derived from human WASL Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.|
Assay Dilutions Overview
Images And Assay Conditions
Figure 1. Western blot analysis of WASL using anti-WASL antibody on HeLa cell lysate(M05438).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASL antigen affinity purified polyclonal antibody (Catalog # M05438) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WASL.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
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