SKU M05438
Size 100ug
Reactivity Human, Mouse
Clonality Monoclonal
Clone Number ACOB-23
Host Rabbit
Applications IF, WB

Overview

Product Name Anti-WASL Monoclonal Antibody
SKU/Catalog Number M05438
Storage & Handling Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Size 100ug
Description Rabbit IgG Monoclonal antibody for O00401 detection. Tested positive for WB IHC IF/IF FC in Human Mouse
Cite This Product Anti-WASL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05438)
Host Rabbit
Form Liquid
Immunogen A synthesized peptide derived from human WASL Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
Reactivity Human, Mouse

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

Images And Assay Conditions

/M/0/M05438 WASL WB test 1.jpg

Figure 1. Western blot analysis of WASL using anti-WASL antibody on HeLa cell lysate(M05438).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASL antigen affinity purified polyclonal antibody (Catalog # M05438) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WASL.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O00401
Gene Name WASL

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Actin, Actin Binding Proteins, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction, Subcellular Markers, Tags & Cell Markers

*You can search these to find other products in these research areas.

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Order Product (M05438)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

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