Click image to see more details
Product Info Summary
| SKU: | A07445 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-WSB1 Antibody
SKU/Catalog Number
A07445
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WSB1 Antibody (Catalog # A07445). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
WSB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-WSB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07445)
Host
Rabbit
Contents
WSB1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1. The immunogen is located within amino acids 70 - 120 of WSB1.
Cross-reactivity
WSB1 antibody is human specific.
Reactive Species
A07445 is reactive to WSB1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
47.4 kDa
Background of WSB1
The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07445 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of WSB1 in human colon tissue lysate with WSB1 antibody at 1 μg/ml.
Click image to see more details
Immunohistochemistry of WSB1 in human colon tissue with WSB1 antibody at 5 μg/mL.
Click image to see more details
Immunofluorescence of WSB1 in human colon tissue with WSB1 antibody at 20 μg/mL.
Specific Publications For Anti-WSB1 Antibody (A07445)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-WSB1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-WSB1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
