Product Info Summary
SKU: | M01884-2 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Xanthine Oxidase Rabbit Monoclonal Antibody
View all Xanthine Oxidase Antibodies
SKU/Catalog Number
M01884-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Xanthine Oxidase Rabbit Monoclonal Antibody catalog # M01884-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Xanthine Oxidase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01884-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19X78
Isotype
IgG
Immunogen
A synthesized peptide derived from human Xanthine Oxidase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01884-2 is reactive to XDH in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
146 kDa
Calculated molecular weight
42616 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01884-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: 293T cell, rat liver tissue, mouse liver tissue
IHC: mouse small intestine tissue, mouse small intestine tissue, rat small intestine tissue, rat small intestine tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of XDH using anti-XDH antibody (M01884-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XDH antigen affinity purified monoclonal antibody (Catalog # M01884-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XDH at approximately 146 kDa. The expected band size for XDH is at 146 kDa.
Click image to see more details
Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
Xanthine Oxidase was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
Xanthine Oxidase was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
Xanthine Oxidase was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
Xanthine Oxidase was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Western blot analysis of Xanthine Oxidase expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For XDH (Source: Uniprot.org, NCBI)
Gene Name
XDH
Full Name
Xanthine dehydrogenase/oxidase
Weight
42616 MW
Superfamily
xanthine dehydrogenase family
Alternative Names
Adiponectin receptor protein 1;Progestin and adipoQ receptor family member I;ADIPOR1;PAQR1 , TESBP1A;CGI-45; XDH XAN1, XO, XOR xanthine dehydrogenase xanthine dehydrogenase/oxidase|xanthine oxidoreductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XDH, check out the XDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Xanthine Oxidase Rabbit Monoclonal Antibody (M01884-2)
Hello CJ!
M01884-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells
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