Anti-YAP1/Yap Rabbit Monoclonal Antibody

Product Name

Anti-YAP1/Yap Rabbit Monoclonal Antibody

View all YAP1 Antibodies

SKU/Catalog Number

M00116

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-YAP1/Yap Rabbit Monoclonal Antibody catalog # M00116. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-YAP1/Yap Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00116)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CIE-25

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human YAP1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00116 is reactive to YAP1 in Human

Applications

M00116 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

54.462kDa

Background of YAP1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of YAP1 using anti-YAP1 antibody (M00116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified monoclonal antibody (Catalog # M00116) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 70 kDa. The expected band size for YAP1 is at 54 kDa.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human uterus, using YAP1 Antibody.

Click image to see more details

Immunofluorescent analysis of MCF7 cells, using YAP1 Antibody .

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For YAP1 (Source: Uniprot.org, NCBI)

Alternative Names

65 kDa Yes-associated protein; YAP; YAP1; YAP2; YAP65; YAP65yes-associated protein 2; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein beta; yes-associated protein delta; YKI; Yorkie Homolog YAP1 COB1, YAP, YAP2, YAP65, YKI Yes1 associated transcriptional regulator transcriptional coactivator YAP1|65 kDa Yes-associated protein|Yes associated protein 1|protein yorkie homolog|yes-associated protein 2|yes-associated protein YAP65 homolog|yorkie homolog

For more info on YAP1, check out the YAP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for YAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the YAP1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis