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PIK3CB antibody (AZE7F251) performs well in IF, IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251).
Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish Kinase p110 Beta/PIK3CB Antibody
PIK3CB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC |
Tested Applications
PIK3CB antibody was positively detected by IHC in:
zebrafish spinal cord tissue, zebrafish brain tissue, zebrafish colon tissue, zebrafish testis tissue, zebrafish kidney tissue, zebrafish muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PIK3CB antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish Kinase p110 Beta/PIK3CB Antibody
View all PIK3CB Antibodies
View all PIK3CB Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish PIK3CB recombinant protein (Position: E562-L611).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of PIK3CB
Predicted to enable 1-phosphatidylinositol-3-kinase activity and 1-phosphatidylinositol-4-phosphate 3-kinase activity. Predicted to be involved in cell migration; intracellular signal transduction; and phosphatidylinositol-3-phosphate biosynthetic process. Predicted to act upstream of or within phosphatidylinositol phosphate biosynthetic process and phosphorylation. Predicted to be part of phosphatidylinositol 3-kinase complex. Predicted to be active in cytoplasm and plasma membrane. Human ortholog(s) of this gene implicated in glioblastoma; prostate adenocarcinoma; prostate cancer; and type 2 diabetes mellitus. Orthologous to human PIK3CB (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta).
Cite This Product
Anti-Zebrafish Kinase p110 Beta/PIK3CB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZE7F251)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Rabbit
88%
Moderate Homology
Dog
88%
Moderate Homology
Human
86%
Moderate Homology
Monkey
86%
Moderate Homology
Cow
84%
Moderate Homology
Sheep
84%
Moderate Homology
Drosophila
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for PIK3CB antibody
Immunofluorescence (IF) protocol for PIK3CB antibody
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Product Promise
We promise Anti-Zebrafish Kinase p110 Beta/PIK3CB Antibody performs excellently in relevant applications for IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZE7F251
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026