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Product Info Summary
| SKU: | A03300-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZIPK DAPK3 Antibody
View all DAPK3/ZIPK Antibodies
SKU/Catalog Number
A03300-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIPK DAPK3 Antibody (Catalog # A03300-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZIPK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIPK DAPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03300-1)
Host
Rabbit
Contents
ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Reactive Species
A03300-1 is reactive to DAPK3 in Human, Mouse, Rat
Applications
A03300-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.536kDa
Background of DAPK3/ZIPK
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.
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Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
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Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DAPK3 (Source: Uniprot.org, NCBI)
Gene Name
DAPK3
Full Name
Death-associated protein kinase 3
Weight
52.536kDa
Superfamily
protein kinase superfamily
Alternative Names
DAP kinase 3; DAPK3; DAP-like kinase; death-associated protein kinase 3; Dlk; EC 2.7.11; EC 2.7.11.1; ZIP kinase isoform; ZIP; ZIPK; ZIPKFLJ36473; ZIP-kinase DAPK3 DLK, ZIP, ZIPK death associated protein kinase 3 death-associated protein kinase 3|DAP kinase 3|DAP-like kinase|MYPT1 kinase|ZIP-kinase|zipper-interacting protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAPK3, check out the DAPK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIPK DAPK3 Antibody (A03300-1)
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Customer Reviews
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