Click image to see more details
Product Info Summary
| SKU: | A04433-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ZNF148/Zbp 89 Antibody
SKU/Catalog Number
A04433-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZNF148/Zbp 89 Antibody (Catalog # A04433-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNF148/Zbp 89 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04433-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF148.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04433-1 is reactive to ZNF148 in Human, Mouse, Rat
Applications
A04433-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
88.976kDa
Background of ZNF148
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Boopathi E., J. Biol. Chem. 279:35242-35254(2004).
Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using ZNF148 antibody A04433-1.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZNF148 antibody A04433-1.
Protein Target Info & Infographic
Gene/Protein Information For ZNF148 (Source: Uniprot.org, NCBI)
Gene Name
ZNF148
Full Name
Zinc finger protein 148
Weight
88.976kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
BERF-1; BFCOL1; CACCC box-binding protein; CLL-associated antigen KW-10; HT-BETA; pHZ-52; Transcription factor ZBP-89; ZBP89; ZBP-89; ZFP148zinc finger protein 148 (pHZ-52); Zinc finger DNA-binding protein 89; zinc finger protein 148 ZNF148 BERF-1, BFCOL1, GDACCF, HT-BETA, ZBP-89, ZFP148, pHZ-52 zinc finger protein 148 zinc finger protein 148|CACCC box-binding protein|CLL-associated antigen KW-10|transcription factor ZBP-89|zinc finger DNA-binding protein 89
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF148, check out the ZNF148 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF148: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF148/Zbp 89 Antibody (A04433-1)
Hello CJ!
No publications found for A04433-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ZNF148/Zbp 89 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-ZNF148/Zbp 89 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-ZNF148/Zbp 89 Antibody
Question
We are currently using anti-ZNF148/Zbp 89 antibody A04433-1 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-ZNF148/Zbp 89 antibody (A04433-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
I was wanting to use your anti-ZNF148/Zbp 89 antibody for IHC for rat b-cell kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat b-cell kidney identification?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can see on the product datasheet, A04433-1 anti-ZNF148/Zbp 89 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat b-cell kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
I am looking for to test anti-ZNF148/Zbp 89 antibody A04433-1 on rat b-cell kidney for research purposes, then I may be interested in using anti-ZNF148/Zbp 89 antibody A04433-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The products we sell, including anti-ZNF148/Zbp 89 antibody A04433-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-19
Question
I have a question about product A04433-1, anti-ZNF148/Zbp 89 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04433-1 anti-ZNF148/Zbp 89 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Would anti-ZNF148/Zbp 89 antibody A04433-1 work for IHC with b-cell kidney?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of b-cell kidney, ZNF148 is highly expressed in b-cell kidney. So, it is likely that anti-ZNF148/Zbp 89 antibody A04433-1 will work for IHC with b-cell kidney.
Boster Scientific Support
Answered: 2019-09-26
Question
Please see the WB image, lot number and protocol we used for b-cell kidney using anti-ZNF148/Zbp 89 antibody A04433-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-12
Question
I see that the anti-ZNF148/Zbp 89 antibody A04433-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-15
Question
Would anti-ZNF148/Zbp 89 antibody A04433-1 work on canine IHC with female reproductive system?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Our lab technicians have not tested anti-ZNF148/Zbp 89 antibody A04433-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ZNF148/Zbp 89 antibody A04433-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine female reproductive system in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-13
Question
Is a blocking peptide available for product anti-ZNF148/Zbp 89 antibody (A04433-1)?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
We do provide the blocking peptide for product anti-ZNF148/Zbp 89 antibody (A04433-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-31
Question
Do you have a BSA free version of anti-ZNF148/Zbp 89 antibody A04433-1 available?
J. Jha
Verified customer
Asked: 2018-11-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ZNF148/Zbp 89 antibody A04433-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-16
Question
Would A04433-1 anti-ZNF148/Zbp 89 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
You can see on the product datasheet, A04433-1 anti-ZNF148/Zbp 89 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-27
Question
Is this A04433-1 anti-ZNF148/Zbp 89 antibody reactive to the isotypes of ZNF148?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
The immunogen of A04433-1 anti-ZNF148/Zbp 89 antibody is Synthesized peptide derived from internal of human ZNF148. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-13
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell kidney using anti-ZNF148/Zbp 89 antibody A04433-1. Let me know if you need anything else.
B. Li
Verified customer
Asked: 2016-08-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-16
