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Product Info Summary
| SKU: | EKC1062 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
BL-CAM Colorimetric Cell-Based ELISA Kit
See all Siglec-2/CD22 products
SKU/Catalog Number
EKC1062
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The BL-CAM Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BL-CAM protein expression profile in cells. The kit can be used for measuring the relative amounts of BL-CAM in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BL-CAM.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
BL-CAM Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1062)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1062 is reactive to CD22 in Human samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1062 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-CD22 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells
Protein Target Info & Infographic
Gene/Protein Information For CD22 (Source: Uniprot.Org, NCBI)
Gene Name
CD22
Full Name
B-cell receptor CD22
Weight
95.348kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD22, check out the CD22 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BL-CAM Colorimetric Cell-Based ELISA Kit (EKC1062)
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