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- Table of Contents
Designing a Western blot for SI? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SI Western Blot GuideFacts about Sucrase-isomaltase, intestinal.
.
| Human | |
|---|---|
| Gene Name: | SI |
| Uniprot: | P14410 |
| Entrez: | 6476 |

| Belongs to: |
|---|
| glycosyl hydrolase 31 family |

EC 3.2.1.20; MGC131621; MGC131622; oligosaccharide alpha-1,6-glucosidase; sucrase-isomaltase (alpha-glucosidase); sucrase-isomaltase; sucrase-isomaltase, intestinal
Mass (kDA):
209.453 kDA

| Human | |
|---|---|
| Location: | 3q26.1 |
| Sequence: | 3; NC_000003.12 (164978898..165078496, complement) |
Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
Apical cell membrane; Single-pass type II membrane protein. Brush border.




PMID: 1353958 by Chantret I., et al. Sequence of the complete cDNA and the 5' structure of the human sucrase-isomaltase gene. Possible homology with a yeast glucoamylase.
PMID: 2962903 by Green F., et al. Isolation of a cDNA probe for a human jejunal brush-border hydrolase, sucrase-isomaltase, and assignment of the gene locus to chromosome 3.